Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

122
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

79
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Investigating the Effect of TNF a (-863) and TNFa (-308) genes Polymorphism on the Progression of Disease in Patients with Cystic Fibrosis

Pages

  10335-10341

Abstract

 Background: Recent studies have shown that the course of Cystic fibrosis in patients with this disease differs despite the same mutation in CFTR gene. We aimed to investigate the role of polymorphism in TNF α (-308) and TNF α (-863), and its effect on the phenotype of the patients with Cystic fibrosis and progression of disease. Materials and Methods: In this case-control study, 50 children with Cystic fibrosis and 50 healthy children were examined for TNF-α-308 GA and TNF α-863CA polymorphism. Four ml of citrated blood was taken from the patients in order to perform the DNA purifying and PCR-RFLP. With custom designed primers, PCR was done. Then with restriction enzymes PCR-RFLP was performed on the product of previous PCR. Changes were analyzed taking the following into consideration: diagnosis age, starting point of the pulmonary disease. Hb O2 saturation level, FEV1, and FVC. Also, for each of them, a Schwachman index basis score was calculated and results are mentioned. Results: Patient’ s average age was 21± 5. 1 years old (ranged 5-26 years), and 48. 9% (n=24) of patients were females. The average age at diagnosis was also 39. 78 ± 13. 51 months. Patients with genotype TNF-α-308GA were older in diagnostic time compared to TNF-α-308GG genotype. However, for other variables, such as O2 sat, FEV1, FVC no difference was observed. Patients Heterozygote genotypes for “ C” allele (CA) of TNF-863 have better Schwachman score than CC genotype. Conclusion The results of this research emphasize the importance of genetic factors affecting inflammatory processes. Identifying these variables is helpful in treating patients with Cystic fibrosis disease.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    HASSANZAD, MARYAM, Hajiesmail, Golnaz, Ghanavi, Jalaledin, Khanbabayi, Ghamartaj, Khanbabayi, Ghamartaj, FESHANGCHI BONAB, Mohammad, Razeghi, Ehsan, & VELAYATI, ALI AKBAR. (2019). Investigating the Effect of TNF a (-863) and TNFa (-308) genes Polymorphism on the Progression of Disease in Patients with Cystic Fibrosis. INTERNATIONAL JOURNAL OF PEDIATRICS, 7(11 (71)), 10335-10341. SID. https://sid.ir/paper/765498/en

    Vancouver: Copy

    HASSANZAD MARYAM, Hajiesmail Golnaz, Ghanavi Jalaledin, Khanbabayi Ghamartaj, Khanbabayi Ghamartaj, FESHANGCHI BONAB Mohammad, Razeghi Ehsan, VELAYATI ALI AKBAR. Investigating the Effect of TNF a (-863) and TNFa (-308) genes Polymorphism on the Progression of Disease in Patients with Cystic Fibrosis. INTERNATIONAL JOURNAL OF PEDIATRICS[Internet]. 2019;7(11 (71)):10335-10341. Available from: https://sid.ir/paper/765498/en

    IEEE: Copy

    MARYAM HASSANZAD, Golnaz Hajiesmail, Jalaledin Ghanavi, Ghamartaj Khanbabayi, Ghamartaj Khanbabayi, Mohammad FESHANGCHI BONAB, Ehsan Razeghi, and ALI AKBAR VELAYATI, “Investigating the Effect of TNF a (-863) and TNFa (-308) genes Polymorphism on the Progression of Disease in Patients with Cystic Fibrosis,” INTERNATIONAL JOURNAL OF PEDIATRICS, vol. 7, no. 11 (71), pp. 10335–10341, 2019, [Online]. Available: https://sid.ir/paper/765498/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top