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Information Journal Paper

Title

Monoclonal Antibody Against ROR1 Induces Apoptosis in Human Bladder Carcinoma Cells

Pages

  165-171

Abstract

 Background: Receptor tyrosine kinase-like Orphan Receptor 1 (ROR1) is one of the promising cell surface antigens for targeting cancer cells. The aim of this study was to evaluate ROR1 cell surface expression in Bladder cancer cells using a murine anti-ROR1 Monoclonal antibody (mAb) called 5F1-B10 as well as investigate its potential in apoptosis induction. Methods: Expression of ROR1 in two human bladder cell lines, 5637 and EJ138, as well as a non-cancerous human cell line, Human Fetal Foreskin Fibroblast (HFFF), was examined by Flow cytometry and immunocytochemistry. Immunohistochemical staining of cancer and normal bladder tissues was also performed. Results: The Flow cytometry results showed that 5F1-B10 mAb could recognize ROR1 molecules in 86. 1% and 45. 6% of 5637 and EJ138 cells, respectively. The expression level of ROR1 was 5. 49% in HFFF cells. The immunocytochemistry and immunohistochemistry staining results also confirmed the presence of ROR1 on the surface of both Bladder cancer cells and tissues, respectively. The obtained data from apoptosis assay demonstrated that 5F1-B10 mAb could induce apoptosis in both 5637 and EJ138 cell lines. Conclusion: Taken together, our finding indicates the role of ROR1 in Bladder cancer cell survival and suggests this receptor might be a promising target for developing novel therapeutic agents against bladder carcinoma.

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    APA: Copy

    BAYAT, ALI AHMAD, Sadeghi, Niloufar, FATEMI, RAMINA, NOWROOZI, MOHAMMAD REZA, OHADIAN MOGHADAM, SOLMAZ, Borzuee, Mohadeseh, RADMANESH, AMIN, KHODADOOST, MAHMOOD, SARRAFZADEH, ALI REZA, ZAREI, OMID, & RABBANI, HODJATTALLAH. (2020). Monoclonal Antibody Against ROR1 Induces Apoptosis in Human Bladder Carcinoma Cells. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 12(3), 165-171. SID. https://sid.ir/paper/766109/en

    Vancouver: Copy

    BAYAT ALI AHMAD, Sadeghi Niloufar, FATEMI RAMINA, NOWROOZI MOHAMMAD REZA, OHADIAN MOGHADAM SOLMAZ, Borzuee Mohadeseh, RADMANESH AMIN, KHODADOOST MAHMOOD, SARRAFZADEH ALI REZA, ZAREI OMID, RABBANI HODJATTALLAH. Monoclonal Antibody Against ROR1 Induces Apoptosis in Human Bladder Carcinoma Cells. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2020;12(3):165-171. Available from: https://sid.ir/paper/766109/en

    IEEE: Copy

    ALI AHMAD BAYAT, Niloufar Sadeghi, RAMINA FATEMI, MOHAMMAD REZA NOWROOZI, SOLMAZ OHADIAN MOGHADAM, Mohadeseh Borzuee, AMIN RADMANESH, MAHMOOD KHODADOOST, ALI REZA SARRAFZADEH, OMID ZAREI, and HODJATTALLAH RABBANI, “Monoclonal Antibody Against ROR1 Induces Apoptosis in Human Bladder Carcinoma Cells,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 12, no. 3, pp. 165–171, 2020, [Online]. Available: https://sid.ir/paper/766109/en

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