IDENTIFICATION OF MALASSEZIA SPECIES USING PCR-RFLP MOLECULAR METHOD IN THE PATIENTS WITH PITYRIASIS VERSICOLOR

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HOSSEINI BAFGHI M.; MOZAFARI N.A.; FATA A.M.; NASERI A.; ZARRINFAR H.

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Journal: SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES Year:2017 | Volume:22 | Issue:3 Page(s): 85-93

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Title

IDENTIFICATION OF MALASSEZIA SPECIES USING PCR-RFLP MOLECULAR METHOD IN THE PATIENTS WITH PITYRIASIS VERSICOLOR

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Keywords

MALASSEZIAQ3

PITYRIASIS VERSICOLORQ2

PCR-RFLPQ2

Abstract

Background and Aim: MALASSEZIA yeast can become pathogenic under certain conditions in humans.MALASSEZIA has various species which can cause a number of skin diseases, such as PITYRIASIS VERSICOLOR (tinea versicolor), seborrheic dermatitis and folliculitis and even systemic infections. In the present study, MALASSEZIA species isolated from the patients with PITYRIASIS VERSICOLOR were identified by PCR-PFLP molecular method.Material and Methods: In this study, the scraping specimens of the trunk and scalp of the patients with PITYRIASIS VERSICOLOR were cultured on Dixon agar medium. Finally, one-hundred MALASSEZIAcolonies were obtained. The genomic DNA was extracted by phenol–chloroform method and then was studied by use of PCR-PFLP molecular method. D1-D2 segment in the area of 26srDNA of ITS gene was proliferated by specific primers and then the PCR products were exposed toCfoI restrictive enzyme.Results: Among 100 MALASSEZIA colonies, the most common MALASSEZIA species were; M.globosa(44%), M. globosa / M. restricta (27%), M. restricta (11%), M. sympodialis (7%), M.sympodialis/ M. restricta (4%), M. globosa / M. restricta/M. furfur (1%), M. sympodialis / M.restricta/ M. globosa(1%) and unknown species (5%).Conclusion: The dominant species isolated from patients with PITYRIASIS VERSICOLOR were M.globosa, M. restricta and M. sympodialis, respectively. Thirty-three percent of the specimens had more than oneMALASSEZIA species. Therefore, rapid PCR-RFLP method is recommended for identification ofMALASSEZIA species in epidemiological studies and production of more effective medicines.

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APA: Copy

HOSSEINI BAFGHI, M., MOZAFARI, N.A., FATA, A.M., NASERI, A., & ZARRINFAR, H.. (2017). IDENTIFICATION OF MALASSEZIA SPECIES USING PCR-RFLP MOLECULAR METHOD IN THE PATIENTS WITH PITYRIASIS VERSICOLOR. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 22(3 ), 85-93. SID. https://sid.ir/paper/76707/en

Vancouver: Copy

HOSSEINI BAFGHI M., MOZAFARI N.A., FATA A.M., NASERI A., ZARRINFAR H.. IDENTIFICATION OF MALASSEZIA SPECIES USING PCR-RFLP MOLECULAR METHOD IN THE PATIENTS WITH PITYRIASIS VERSICOLOR. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;22(3 ):85-93. Available from: https://sid.ir/paper/76707/en

IEEE: Copy

M. HOSSEINI BAFGHI, N.A. MOZAFARI, A.M. FATA, A. NASERI, and H. ZARRINFAR, “IDENTIFICATION OF MALASSEZIA SPECIES USING PCR-RFLP MOLECULAR METHOD IN THE PATIENTS WITH PITYRIASIS VERSICOLOR,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 3 , pp. 85–93, 2017, [Online]. Available: https://sid.ir/paper/76707/en

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