IDENTIFICATION OF P FIMBRIAE VIRULENCE FACTOR IN UROPATHOGENIC ESCHERICHIA COLI BY PCR IN SHAHREKORD HOSPITALS

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SERAJIAN A.A.; ZAMANZAD B.; AFROGH P.; SOLTAN DALLAL M.M.

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Journal Paper

Paper Information

Journal: Scientific Journal of Kurdistan University of Medical Sciences Year:2012 | Volume:17 | Issue:2 (SERIAL NUMBER 64) Page(s): 36-43

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Title

IDENTIFICATION OF P FIMBRIAE VIRULENCE FACTOR IN UROPATHOGENIC ESCHERICHIA COLI BY PCR IN SHAHREKORD HOSPITALS

Author(s)

SERAJIAN A.A. | ZAMANZAD B. | AFROGH P. | SOLTAN DALLAL M.M. | Issue Writer Certificate

Keywords

URINARY TRACT INFECTIONQ2

PAP OPERONQ2

PAP C GENEQ2

P FIMBRIAEQ2

Abstract

Background and Aim: URINARY TRACT INFECTION is one of the most common bacterial diseases in human being. The most important etiologic agent is Eschrichia coli & the most important virulence factor in uropathogenic E.coli is P fimberiae. Construction of P FIMBRIAE is coded by PAP OPERON. The importance of P FIMBRIAE is related to the incidence of URINARY TRACT INFECTION, especially infection of the upper tracts. The aim of this study was to identify & determine P FIMBRIAE prevalence in isolated uroparhogenic E.coli.Material and Methods: A total of 182 isolates of E.coli were evaluated .Bacterial DNA was extracted by boiling method & subsequently DNA samples were examined for PAP OPERON (pap C gene) by use of PCR method. Then PCR products were stained and identified by polyacrylamid gel electrophoresis.Results: Based on the results of PCR, among 182 isolated strains, 66 samples (36.3%) had PAP OPERON(pap C gene).Conclusion: The results indicated that prevalence of PAP OPERON in isolated strains was within the global range. Pyelonephritis was more common than cystitis & asympthomatic bacteriuria.

Cites

THE FREQUENCY OF FIMBRIAE GENES PAPA, FIMH, KPSMT, PAPEF AND IBEA IN ESCHERICHIA COLI ISOLATED FROM CHILDREN URINARY TRACT INFECTION

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APA: Copy

SERAJIAN, A.A., ZAMANZAD, B., AFROGH, P., & SOLTAN DALLAL, M.M.. (2012). IDENTIFICATION OF P FIMBRIAE VIRULENCE FACTOR IN UROPATHOGENIC ESCHERICHIA COLI BY PCR IN SHAHREKORD HOSPITALS. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 17(2 (SERIAL NUMBER 64)), 36-43. SID. https://sid.ir/paper/76795/en

Vancouver: Copy

SERAJIAN A.A., ZAMANZAD B., AFROGH P., SOLTAN DALLAL M.M.. IDENTIFICATION OF P FIMBRIAE VIRULENCE FACTOR IN UROPATHOGENIC ESCHERICHIA COLI BY PCR IN SHAHREKORD HOSPITALS. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;17(2 (SERIAL NUMBER 64)):36-43. Available from: https://sid.ir/paper/76795/en

IEEE: Copy

A.A. SERAJIAN, B. ZAMANZAD, P. AFROGH, and M.M. SOLTAN DALLAL, “IDENTIFICATION OF P FIMBRIAE VIRULENCE FACTOR IN UROPATHOGENIC ESCHERICHIA COLI BY PCR IN SHAHREKORD HOSPITALS,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 17, no. 2 (SERIAL NUMBER 64), pp. 36–43, 2012, [Online]. Available: https://sid.ir/paper/76795/en

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