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Information Journal Paper

Title

Inhibition of miR-200b Promotes Angiogenesis in Endothelial Cells by Activating The Notch Pathway

Pages

  51-60

Abstract

 Objective: Patients with diabetes mellitus frequently have chronic wounds or diabetic ulcers as a result of impaired wound healing, which may lead to limb amputation. Human umbilical vein endothelial cell (HUVEC) dysfunction also delays wound healing. Here, we investigated the mechanism of miR-200b in HUVECs under high glucose conditions and the potential of miR-200b as a therapeutic target. Materials and Methods: In this experimental study, HUVECs were cultured with 5 or 30 mM glucose for 48 hours. Cell proliferation was evaluated by CCK-8 assays. Cell mobility was tested by wound healing and Transwell assays. Angiogenesis was analyzed in vitro Matrigel tube formation assays. Luciferase reporter assays were used to test the binding of miR-200b with Notch1. Results: miR-200b expression was induced by high glucose treatment of HUVECs (P<0. 01), and it significantly repressed cell proliferation, migration, and tube formation (P<0. 05). Notch1 was directly targeted and repressed by miR-200b at both the mRNA and protein levels. Inhibition of miR-200b restored Notch1 expression (P<0. 05) and reactivated the Notch Pathway. The effects of miR-200b inhibition in HUVECs could be reversed by treatment with a Notch Pathway inhibitor (P<0. 05), indicating that the miR-200b/Notch axis modulates the proliferation, migration, and tube formation ability of HUVECs. Conclusion: Inhibition of miR-200b activated the angiogenic ability of endothelial cells and promoted wound healing through reactivation of the Notch Pathway in vitro. miR-200b could be a promising therapeutic target for treating HUVEC Dysfunction.

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    • Cite

    APA: Copy

    Qiu, Tie Ying, HUANG, JIN, Wang, Li Ping, & Zhu, Bi Song. (2021). Inhibition of miR-200b Promotes Angiogenesis in Endothelial Cells by Activating The Notch Pathway. CELL JOURNAL (YAKHTEH), 23(1 (89)), 51-60. SID. https://sid.ir/paper/772825/en

    Vancouver: Copy

    Qiu Tie Ying, HUANG JIN, Wang Li Ping, Zhu Bi Song. Inhibition of miR-200b Promotes Angiogenesis in Endothelial Cells by Activating The Notch Pathway. CELL JOURNAL (YAKHTEH)[Internet]. 2021;23(1 (89)):51-60. Available from: https://sid.ir/paper/772825/en

    IEEE: Copy

    Tie Ying Qiu, JIN HUANG, Li Ping Wang, and Bi Song Zhu, “Inhibition of miR-200b Promotes Angiogenesis in Endothelial Cells by Activating The Notch Pathway,” CELL JOURNAL (YAKHTEH), vol. 23, no. 1 (89), pp. 51–60, 2021, [Online]. Available: https://sid.ir/paper/772825/en

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