L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

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Ezati Daniyal; Vardiyan Reyhane; TALEBI ALI REZA; ANVARI MORTEZA; POURENTEZARI MAJID

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Journal: International Journal of Reproductive BioMedicine Year:2020 | Volume:18 | Issue:10 Page(s): 837-846

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Title

L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

Author(s)

Keywords

Formalin

L-carnitine

Mice

Sperm chromatin

Apoptosis

Abstract

Background: Formalin is commonly applied as an antiseptic and tissue fixative. It has reactive molecules that lead to its cytotoxic effects. According to recent studies, Formalin causes a change in the testicular and sperm structure and L-carnitine (LC) acts as an antioxidant to counteract its effects. Objective: This study aimed to investigate the protective effects of LC on the parameters, chromatin condensation and Apoptosis of Mice sperm exposed to Formalin. Materials and Methods: In this experimental study, 24 balb/c Mice (25-40 gr, 10-12 wk) were divided into three groups (n = 8/each): group I without any injections or gavage; group II, received 10 mg/ kg Formalin intraperitoneally (I. P); and group III was exposed to Formalin and LC, where a dose of 10 mg/kg Formalin was injected I. P daily and LC the dose of 100 mg/kg was kept in a solvent solution. After 31 days, the sperm examination was performed as follows: to evaluate chromatin and DNA quality of the sperm, we applied aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), and terminal transferase-mediated deoxy uridine triphosphate biotin end labeling (TUNEL) tests. Results: Sperm parameters such as count, motility, morphology, and viability displayed a significant decrease in the Formalin group. While the data exhibited a considerable augment in sperm parameters in the Formalin + LC than the Formalin and control groups (p < 0. 001), significant differences were detected between groups with respect to TB staining, TUNEL test, CMA3 test and AB staining in the Formalin and Formalin + LC groups. Conclusion: LC can reduce the negative effects of Formalin on sperm parameters, chromatin stability, and percentage of Apoptosis in an animal model.

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Histological study of the placenta in the rats treated with curcumin and formaldehyde

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APA: Copy

Ezati, Daniyal, Vardiyan, Reyhane, TALEBI, ALI REZA, ANVARI, MORTEZA, & POURENTEZARI, MAJID. (2020). L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 18(10), 837-846. SID. https://sid.ir/paper/775092/en

Vancouver: Copy

Ezati Daniyal, Vardiyan Reyhane, TALEBI ALI REZA, ANVARI MORTEZA, POURENTEZARI MAJID. L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2020;18(10):837-846. Available from: https://sid.ir/paper/775092/en

IEEE: Copy

Daniyal Ezati, Reyhane Vardiyan, ALI REZA TALEBI, MORTEZA ANVARI, and MAJID POURENTEZARI, “L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 18, no. 10, pp. 837–846, 2020, [Online]. Available: https://sid.ir/paper/775092/en

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