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Information Journal Paper

Title

Collagen I Gel Increases the Osteogenic Potential of Platelet-Rich Plasma in Adipose-Derived Stem Cells

Pages

  373-381

Abstract

 Objectives: Adipose-derived mesenchymal stem cells (ASCs) have osteogenic potential. Platelet-rich plasma (PRP) is an alternative natural replacement for osteogenic growth factors. The present study evaluated the combinatory effect of human PRP (hPRP) and collagen I (Col I) gels on the osteogenic potential of ASCs. Materials and Methods: In current experimental research, the extracted ASCs from the pararenal fat pad, at passage 3 were used for the experiments. The osteoinductive potential of ASCs was examined by culturing the cells in cell culture media supplemented with 10% hPRP, 10% Col I, and 10% hPRP/Col I. Finally, metabolic activity, osteoblast differentiation, and mineralization were assessed through the MTT method, alkaline phosphatase assay, Von Kossa method, and staining of osteocalcin (OCN) immunocytochemistry, respectively. Results: Based on the results, 10% hPRP gel, 10% Col I gel, and 10% hPRP/Col I gel increased the metabolic activity and proliferation of ASCs (P < 0. 05). In addition, the activity of alkaline phosphatase in ASCs, supplemented with 10% hPRP/Col I gel was extremely higher compared to the other groups on days 7 and 14 (P < 0. 05). Further, calcified nodules were evident on day 14 after the osteogenic stimulation of ASCs which were cultured in 10% hPRP/Col I gel. Eventually, positive OCN expression was detected in 10% hPRP/Col I gel on days 7 and 14. Conclusions: These findings indicated that the combination of hPRP and Col I gels provides a natural biomaterial for increasing the proliferation and osteoblast differentiation of ASCs.

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    APA: Copy

    Kalarestaghy, Hossein, SHAFAEI, HAJAR, Farahzadi, Raheleh, VAHEDI, PARVIZ, Dolathkhah, Mohamad Amin, DELAZAR, ABBAS, & KARIMIAN FATHI, NAHID. (2020). Collagen I Gel Increases the Osteogenic Potential of Platelet-Rich Plasma in Adipose-Derived Stem Cells. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, 7(3), 373-381. SID. https://sid.ir/paper/775324/en

    Vancouver: Copy

    Kalarestaghy Hossein, SHAFAEI HAJAR, Farahzadi Raheleh, VAHEDI PARVIZ, Dolathkhah Mohamad Amin, DELAZAR ABBAS, KARIMIAN FATHI NAHID. Collagen I Gel Increases the Osteogenic Potential of Platelet-Rich Plasma in Adipose-Derived Stem Cells. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES[Internet]. 2020;7(3):373-381. Available from: https://sid.ir/paper/775324/en

    IEEE: Copy

    Hossein Kalarestaghy, HAJAR SHAFAEI, Raheleh Farahzadi, PARVIZ VAHEDI, Mohamad Amin Dolathkhah, ABBAS DELAZAR, and NAHID KARIMIAN FATHI, “Collagen I Gel Increases the Osteogenic Potential of Platelet-Rich Plasma in Adipose-Derived Stem Cells,” CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, vol. 7, no. 3, pp. 373–381, 2020, [Online]. Available: https://sid.ir/paper/775324/en

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