CHARACTERIZATION OF LEISHMANIA PARASITES ISOLATED FROM KALA- AZAR PATIENTS IN KOHGILOYEH AND BOYERAHMAD, USING SEMI-NESTED PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SARKARI B.; FAKHAR M.; EBRAHIMI S.; MOTAZEDIAN M.H.; HATAM GH.; KALANTARI M.; REZANEZHAD H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Armaghane Danesh Year:2006 | Volume:11 | Issue:1 (41) Page(s): 27-34

Download Full-Text

Persian Verion

View:

899

Download:

Cites:

Information Journal Paper

Title

CHARACTERIZATION OF LEISHMANIA PARASITES ISOLATED FROM KALA- AZAR PATIENTS IN KOHGILOYEH AND BOYERAHMAD, USING SEMI-NESTED PCR

Author(s)

Keywords

LEISHMANIAQ3

KALA AZARQ3

SEMI-NESTED PCRQ3

Abstract

Introduction & Objective: Visceral LEISHMANIAsis (VL) is a disease commonly known as Kala-azar caused by protozoan parasites of the genus LEISHMANIA including L. donovani, L. infantum and L. chagasi. VL is sporadic in manyareas of Iran and is endemic in a few provinces such as Fars, Azarbayjan, Bushehr, Ardabil and Qom. VL has been reported from some areas of Kohgiloyeh and Boyerahmad and this study aimed to characterize the causative agent of VL in this region. Materials & Methods: Bone marrow sample was obtained from 6 VL patients from children department in Imam Sajad hospital in Yasuj. DNA was extracted from the obtained samples and was checked by SEMI-NESTED PCR to determine the species of the parasite. To do that, a segment of minicircle kinetoplast DNA was amplified, using LINR4 and LIN17 primers. Products of PCR were evaluated by electrophoresis, using 1.5% agarose and stained with ethidium bromide. Results: Parasitologically examination of bone marrow smears demonstrated amastigotes form of the parasite in the samples. For mass cultivation, isolated parasites were cultured in diphasic NNN followed by RPMI 1640 media. All the samples produced a 720 bp band in PCR assay. The isolates were compared with referent strains and it was revealed that all the isolates were L. infantum. Conclusion: Findings of this study demonstrated that the causative agent of VL in Kohgiloyeh and Boyerahmad was L. infantum. Further study is needed to explore other aspects of VL in this region.

Cites

SEROLOGICAL STUDY OF VISCERAL LEISHMANIASIS IN BOYER AHMAD TOWNSHIP IN 2005

References

No record.

Cite

APA: Copy

SARKARI, B., FAKHAR, M., EBRAHIMI, S., MOTAZEDIAN, M.H., HATAM, GH., KALANTARI, M., & REZANEZHAD, H.. (2006). CHARACTERIZATION OF LEISHMANIA PARASITES ISOLATED FROM KALA- AZAR PATIENTS IN KOHGILOYEH AND BOYERAHMAD, USING SEMI-NESTED PCR. ARMAGHAN DANESH, 11(1 (41)), 27-34. SID. https://sid.ir/paper/77670/en

Vancouver: Copy

SARKARI B., FAKHAR M., EBRAHIMI S., MOTAZEDIAN M.H., HATAM GH., KALANTARI M., REZANEZHAD H.. CHARACTERIZATION OF LEISHMANIA PARASITES ISOLATED FROM KALA- AZAR PATIENTS IN KOHGILOYEH AND BOYERAHMAD, USING SEMI-NESTED PCR. ARMAGHAN DANESH[Internet]. 2006;11(1 (41)):27-34. Available from: https://sid.ir/paper/77670/en

IEEE: Copy

B. SARKARI, M. FAKHAR, S. EBRAHIMI, M.H. MOTAZEDIAN, GH. HATAM, M. KALANTARI, and H. REZANEZHAD, “CHARACTERIZATION OF LEISHMANIA PARASITES ISOLATED FROM KALA- AZAR PATIENTS IN KOHGILOYEH AND BOYERAHMAD, USING SEMI-NESTED PCR,” ARMAGHAN DANESH, vol. 11, no. 1 (41), pp. 27–34, 2006, [Online]. Available: https://sid.ir/paper/77670/en

Related Journal Papers