Presence of Silver Resistance Genes is not associated with Minimum Inhibitory Concentration of Silver Nanoparticles against Staphylococcus aureus and Escherichia coli

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Azadi Fahimeh; JAVID NAEME; BAGHERI HANIEH; GHAEMI EZZAT ALLAH

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Journal: MEDICAL LABORATORY JOURNAL Year:2020 | Volume:14 | Issue:4 Page(s): 7-12

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Title

Presence of Silver Resistance Genes is not associated with Minimum Inhibitory Concentration of Silver Nanoparticles against Staphylococcus aureus and Escherichia coli

Author(s)

Azadi Fahimeh | JAVID NAEME | BAGHERI HANIEH | GHAEMI EZZAT ALLAH | Issue Writer Certificate

Keywords

Staphylococcus aureus

Escherichia coli

Silver particles

MIC

Abstract

Background and objectives: Drug resistance in Staphylococcus aureus and Escherichia coli, as severe pathogenic bacteria, has become a health challenge. However, nanoparticles have been introduced as effective candidates for their eradication. In this study, we investigated presence of genes involved in conferring resistance to silver nanoparticles in S. aureus and E. coli isolates and evaluated its association with minimal inhibitory concentration (MIC) of the nanoparticles against these isolates. Methods: The MIC of silver nanoparticles against 121 clinical isolates of E. coli and 183 S. aureus isolates was assessed by broth MICrodilution assay. Presence and expression of the silver resistance genes (silE, silR/S) in the isolates were investigated by PCR and real-time PCR, respectively. Results: The silE gene was found in three (1. 6%) S. aureus and four (3%) E. coli isolates. MIC of silver nanoparticles against S. aureus isolates with the silE gene was 1, 2 and 8 μ g/ml. Moreover, the MIC of the nanoparticles against silEpositive E. coli isolates was 16 μ g/ml in three cases and 8 μ g/ml in one case. None of the S. aureus isolates contained the silR/S gene, but presence of both silE and silR/S was confirmed in two E. coli isolates. Real-time PCR showed no sil expression in the isolates containing the resistance genes. Conclusion: The frequency of the silver resistance genes among S. aureus and E. coli isolates is very low. There is no relationship between presence of the resistance genes and the MIC value of silver nanoparticles.

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APA: Copy

Azadi, Fahimeh, JAVID, NAEME, BAGHERI, HANIEH, & GHAEMI, EZZAT ALLAH. (2020). Presence of Silver Resistance Genes is not associated with Minimum Inhibitory Concentration of Silver Nanoparticles against Staphylococcus aureus and Escherichia coli. MEDICAL LABORATORY JOURNAL, 14(4), 7-12. SID. https://sid.ir/paper/777303/en

Vancouver: Copy

Azadi Fahimeh, JAVID NAEME, BAGHERI HANIEH, GHAEMI EZZAT ALLAH. Presence of Silver Resistance Genes is not associated with Minimum Inhibitory Concentration of Silver Nanoparticles against Staphylococcus aureus and Escherichia coli. MEDICAL LABORATORY JOURNAL[Internet]. 2020;14(4):7-12. Available from: https://sid.ir/paper/777303/en

IEEE: Copy

Fahimeh Azadi, NAEME JAVID, HANIEH BAGHERI, and EZZAT ALLAH GHAEMI, “Presence of Silver Resistance Genes is not associated with Minimum Inhibitory Concentration of Silver Nanoparticles against Staphylococcus aureus and Escherichia coli,” MEDICAL LABORATORY JOURNAL, vol. 14, no. 4, pp. 7–12, 2020, [Online]. Available: https://sid.ir/paper/777303/en

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