مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

135
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

70
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Molecular Detection of Arcobacter in Human Stool Samples Using Housekeeping Genes

Pages

  75-80

Abstract

 Background: Arcobacter is one of the most common bacteria in humans and livestock, leading to gastroenteritis in humans as well as genital and enteric diseases in animals. This bacterium is known to be the main cause of Diarrhea. In molecular studies, the 16SrRNA gene was primarily used as the standard gene for the determination of the Arcobacter. The purpose of this study was to investigate the molecular detection of Arcobacter using glyA, atpA, and gyrA genes compared to16SrRNA. Methods: In this study, 61 samples of Arcobacter DNA isolated from fecal specimens of patients and healthy individuals in the sample bank were used. In order to detect Arcobacter, the intended primers for 16SrRNA as well as glyA, atpA, and gyrA genes were used for Polymerase chain reaction (PCR). The products obtained from the PCR were sequenced. Results: The results of the proliferation reactions indicated the accuracy of the intended primers and the associated molecular experiments. Our results showed that 65. 57% of the cases were detected to be positive for Arcobacter among 61 samples using the glyA gene. This percentage was higher compared to 16SrRNA (42. 62%), gyrA (42. 62%), and atpA (24. 59%). The analysis was statistically significant. Conclusions: Given the presence of repetitive sequences in the 16SrRNA in most bacteria, the interpretation of the results is likely to be difficult for researchers. The results of this study showed more sensitivity and accurate diagnosis of Arcobacter using the glyA gene than other studied genes. In diagnostic studies of Arcobacter, the glyA gene is proposed as an alternative to the 16SrRNA.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    Karamghoshchi, Aysan, AHMADI, AZAM, ARJOMANDZADEGAN, MOHAMMAD, AKBARI, MAJID, & Ghorbani Marghmaleki, Elahe. (2020). Molecular Detection of Arcobacter in Human Stool Samples Using Housekeeping Genes. AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION, 7(3), 75-80. SID. https://sid.ir/paper/778027/en

    Vancouver: Copy

    Karamghoshchi Aysan, AHMADI AZAM, ARJOMANDZADEGAN MOHAMMAD, AKBARI MAJID, Ghorbani Marghmaleki Elahe. Molecular Detection of Arcobacter in Human Stool Samples Using Housekeeping Genes. AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION[Internet]. 2020;7(3):75-80. Available from: https://sid.ir/paper/778027/en

    IEEE: Copy

    Aysan Karamghoshchi, AZAM AHMADI, MOHAMMAD ARJOMANDZADEGAN, MAJID AKBARI, and Elahe Ghorbani Marghmaleki, “Molecular Detection of Arcobacter in Human Stool Samples Using Housekeeping Genes,” AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION, vol. 7, no. 3, pp. 75–80, 2020, [Online]. Available: https://sid.ir/paper/778027/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button