INFLUENCE OF INSULIN-LIKE GROWTH FACTOR 1 ON NUCLEAR MATURATION OF GERMINAL VESICLE MOUSE OOCYTES

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MAHMOUDI R.; MOUSAVI E.; RABANI S.M.; AKBARTABARTOORI M.; DEHGHANI M.; DEHGHANI F.

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Journal Paper

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Journal: Armaghane Danesh Year:2014 | Volume:19 | Issue:6 (89) Page(s): 487-496

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Title

INFLUENCE OF INSULIN-LIKE GROWTH FACTOR 1 ON NUCLEAR MATURATION OF GERMINAL VESICLE MOUSE OOCYTES

Author(s)

Keywords

OOCYTESQ2

CUMULUS CELLQ3

IN VITRO MATURATIONQ2

INSULIN-LIKE GROWTH FACTORQ2

Abstract

Background & aim: IN VITRO MATURATION and fertilization of OOCYTES play an important role in reproductive biotechnology. The aim of this study is to define the IGF-1 effect on IN VITRO MATURATION, fertilization and development of mice immature OOCYTES to 2-cells in TCM199 medium cultures.Methods: In this study 4 week old NMRI mice were used. Ovaries stimulation carried out using PMSG. GV OOCYTES with or without CUMULUS CELLs were isolated from ovaries and cultured in TCM199 in presence of 100 ng IGF-1 for 24hr.The OOCYTES (MII) were inseminated with sperm in T6 medium for fertilization and development of 2-cells stage and they were investigated under inverted microscope. Data analysis was performed by using Chi- 2 test.Results: In CUMULUS CELL group and in the presence of INSULIN-LIKE GROWTH FACTOR fertilization of OOCYTES, forming embryos and the formation of 2-cells compared to the group without CUMULUS CELLs significantly increased (p<0.05).Conclusion: As the results showed OOCYTES with CUMULUS CELLs in the presence of INSULIN-LIKE GROWTH FACTOR enhances maturation, fertilization and embryonic development in 2-cells OOCYTES compared to group without CUMULUS CELLs TCM199.

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