The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study

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OMIDI ZEINAB; PIRAVAR ZEINAB; RAMEZANI MINA

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Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2020 | Volume:18 | Issue:10 Page(s): 855-864

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Title

The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study

Author(s)

OMIDI ZEINAB | PIRAVAR ZEINAB | RAMEZANI MINA | Issue Writer Certificate

Keywords

Acrylamides

Spermatozoa

Mitochondrial membrane potential

Glutathione S-Transferase

Abstract

Background: Acrylamide (AA) is a compound used in the industrial production of polyacrylamide. AAs affects by creating oxidative stress. It produces reactive oxygen species and leads to lipid peroxide. Lipid peroxidation in the cell membrane is one of the most important oxidations in the sperm, which can disrupt the fluidity and permeability of cell membranes and damage all cells. Objective: To investigate the different concentrations of AA on human sperm parameters based on the World Health Organization standard and its impact on Mitochondrial membrane potential and sperm glutathione levels. Materials and Methods: In this laboratory study, we examined the different concentrations of AA on human sperm parameters based on the World Health Organization standard and its impact on Mitochondrial membrane potential by flow cytometry and sperm glutathione levels by ELISA assay. Results: The results were reported as the mean fluorescence intensity of JC and the index was observed to decrease following the effect of AA in Mitochondrial membrane potential (Δ Ψ m). The results of ELISA test to study the level of intracellular glutathione showed that with the increase in the concentration of AA exposed to sperms, there was a significant reduction in the level of intracellular glutathione. Conclusion: AA destroys the sperm membrane integrity under apoptotic and oxidative inductions with a negative impact on mitochondrial function and antioxidative enzyme in sperm such as glutathione.

Cites

The effect of interval training and Nano selenium supplementation on the expression of testicular HSP70 and PGC-1α,genes in dexamethasone induced rats

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APA: Copy

OMIDI, ZEINAB, PIRAVAR, ZEINAB, & RAMEZANI, MINA. (2020). The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 18(10), 855-864. SID. https://sid.ir/paper/780593/en

Vancouver: Copy

OMIDI ZEINAB, PIRAVAR ZEINAB, RAMEZANI MINA. The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2020;18(10):855-864. Available from: https://sid.ir/paper/780593/en

IEEE: Copy

ZEINAB OMIDI, ZEINAB PIRAVAR, and MINA RAMEZANI, “The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 18, no. 10, pp. 855–864, 2020, [Online]. Available: https://sid.ir/paper/780593/en

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