HIGH-EXPRESSION OF CHIMERIC ANTIGEN RECEPTOR ON T LYMPHOCYTES BY GENOMIC INSERTION

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JAFARI IRI SOFLA F.; RAHBARIZADEH F.; RASAEE M.J.; AGHAII BAKHTIARI H.; KHALEGHI S.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2012 | Volume:9 | Issue:1 (34) Page(s): 8-18

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Title

HIGH-EXPRESSION OF CHIMERIC ANTIGEN RECEPTOR ON T LYMPHOCYTES BY GENOMIC INSERTION

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Keywords

INTEGRASEQ3

CANCERQ3

IMMUNOTHERAPYQ3

Abstract

Background and Objectives: The specific activation of the immune system especially T lymphocytes has been a long-term goal of CANCER IMMUNOTHERAPY. In an approach to induce the patient’s own immune cells, chimeric antigen receptors (CAR) were used to activate cytotoxic T lymphocytes. The CAR consists of an antigen binding domain of antibody molecule linked through an extracellular linker to transmembrane and cytoplasmic domains of lymphocyte-triggering moieties.Materials and Methods: In our research group, variable domains of heavy chain antibodies (VHH or nanobody) derived from camelids were used instead of the antigen-binding domain in chimeric receptors constructs. To obtain a stable and increased expression of VHH harboring CAR, the PhiC31 INTEGRASE system was used for CAR gene integration into the T-cell genome. Constructs expressing CAR and PhiC31 INTEGRASE were co-transfected into the Jurkat cell line and CAR expression was quantified after one day and 30 days after transfection by a semi-quantitative RT-PCR method.Results: Our results on day 30 confirmed that co-transfection of PhiC31 INTEGRASE constructs and CAR expressing constructs resulted in stable and high expression of the receptors.Conclusions: Based on our results, we conclude that PhiC31 INTEGRASE enzyme can be used for IMMUNOTHERAPY of CANCER mediated by T cells expressing chimeric recetors.

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APA: Copy

JAFARI IRI SOFLA, F., RAHBARIZADEH, F., RASAEE, M.J., AGHAII BAKHTIARI, H., & KHALEGHI, S.. (2012). HIGH-EXPRESSION OF CHIMERIC ANTIGEN RECEPTOR ON T LYMPHOCYTES BY GENOMIC INSERTION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 9(1 (34)), 8-18. SID. https://sid.ir/paper/78309/en

Vancouver: Copy

JAFARI IRI SOFLA F., RAHBARIZADEH F., RASAEE M.J., AGHAII BAKHTIARI H., KHALEGHI S.. HIGH-EXPRESSION OF CHIMERIC ANTIGEN RECEPTOR ON T LYMPHOCYTES BY GENOMIC INSERTION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2012;9(1 (34)):8-18. Available from: https://sid.ir/paper/78309/en

IEEE: Copy

F. JAFARI IRI SOFLA, F. RAHBARIZADEH, M.J. RASAEE, H. AGHAII BAKHTIARI, and S. KHALEGHI, “HIGH-EXPRESSION OF CHIMERIC ANTIGEN RECEPTOR ON T LYMPHOCYTES BY GENOMIC INSERTION,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 9, no. 1 (34), pp. 8–18, 2012, [Online]. Available: https://sid.ir/paper/78309/en

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