EXPANSION OF CD34+ UMBILICAL CORD BLOOD IN 3 DIMENTIONAL CULTURE MEDIA ON MESENCHYMAL STEM CELL COATED SCAFFOLD

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RANJBARAN R.; ABOLGHASEMI H.; NASIRI N.; OODI A.; NIKOUGOFTAR M.; AMANI M.; AMIRIZADEH N.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2012 | Volume:9 | Issue:3 (36) Page(s): 177-185

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Title

EXPANSION OF CD34+ UMBILICAL CORD BLOOD IN 3 DIMENTIONAL CULTURE MEDIA ON MESENCHYMAL STEM CELL COATED SCAFFOLD

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Keywords

MESENCHYMAL STEM CELLSQ3

UMBILICAL CORD BLOODQ3

CD34 ANTIGENQ3

STEM CELLSQ3

Abstract

Background and Objectives: Stem cell transplantation has achieved high success for treatment but the use of this source has some limitation. Cord blood transplantation is clinically limited by its low progenitor cell contents such as CD34+. The aim of this study was to evaluate the effect of STEM CELLS/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">MESENCHYMAL STEM CELLS (MSCs) on replication of CD34+ in cord blood.Materials and Methods: In this experimental study MSCs were isolated and cultured from BM mononuclear cells.MSCs were used for co-culture in two systems of 2D and 3D cultures. After isolation, UMBILICAL CORD BLOOD CD34+ cells were cultured in two different systems. The level of expansion in these systems was evaluated by cell count, analysis of CD34+ expression by flowcytometery, and CFU assay.Results: Expansion of cord blood HSCs was evaluated at three different culture systems at three different times (day3, day 7 and day 10): the medium with cytokine, 2D medium with cytokine+MSCs, and 3D medium with cytokine+MSCs on scaffold. The fold increase of TNC, CFU, and CD34+ cells at day 10 for the former medium was 87±6, 84±8, 11±3, for the second 103±8, 111±9, 14±3, and for the latter 127±10, 170±14, 20±4, respectively. The highest expansion was observed in the 3D co-culture with MSCs after 10 days.Conclusions: The results have demonstrated that the co-culture of CB HSCs with mesenchymal cells could increase the rate of expansion especially in the 3D system.

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APA: Copy

RANJBARAN, R., ABOLGHASEMI, H., NASIRI, N., OODI, A., NIKOUGOFTAR, M., AMANI, M., & AMIRIZADEH, N.. (2012). EXPANSION OF CD34⁺ UMBILICAL CORD BLOOD IN 3 DIMENTIONAL CULTURE MEDIA ON MESENCHYMAL STEM CELL COATED SCAFFOLD. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 9(3 (36)), 177-185. SID. https://sid.ir/paper/78325/en

Vancouver: Copy

RANJBARAN R., ABOLGHASEMI H., NASIRI N., OODI A., NIKOUGOFTAR M., AMANI M., AMIRIZADEH N.. EXPANSION OF CD34⁺ UMBILICAL CORD BLOOD IN 3 DIMENTIONAL CULTURE MEDIA ON MESENCHYMAL STEM CELL COATED SCAFFOLD. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2012;9(3 (36)):177-185. Available from: https://sid.ir/paper/78325/en

IEEE: Copy

R. RANJBARAN, H. ABOLGHASEMI, N. NASIRI, A. OODI, M. NIKOUGOFTAR, M. AMANI, and N. AMIRIZADEH, “EXPANSION OF CD34⁺ UMBILICAL CORD BLOOD IN 3 DIMENTIONAL CULTURE MEDIA ON MESENCHYMAL STEM CELL COATED SCAFFOLD,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 9, no. 3 (36), pp. 177–185, 2012, [Online]. Available: https://sid.ir/paper/78325/en

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