INDUCTION OF G1 CELL CYCLE ARREST AND INCREASED SUB-G1 POPULATION UPON TREATMENT OF NALM-6 CELLS WITH SYNTHETIC INHIBITOR OF HTERT

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ZAREII M.; BASHASH D.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2017 | Volume:13 | Issue:4 Page(s): 314-323

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Title

INDUCTION OF G1 CELL CYCLE ARREST AND INCREASED SUB-G1 POPULATION UPON TREATMENT OF NALM-6 CELLS WITH SYNTHETIC INHIBITOR OF HTERT

Author(s)

ZAREII M. | BASHASH D. | Issue Writer Certificate

Keywords

ACUTE LYMPHOID LEUKEMIAQ2

BIBR1532Q2

TELOMERASEQ2

Abstract

Background and Objectives: As the role of TELOMERASE in unlimited proliferation is a common feature of the majority of human cancers including hematological malignancies, thus inhibition of this enzyme has been proposed as a novel strategy in cancer therapeutics. This study was performed to investigate the effect of BIBR1532, a synthetic inhibitor of hTERT, on metabolic activity, DNA synthesis rate, cell cycle activity, and expression of pro-apoptotic genes such as p21, p73, Bax, and Bad in Nalm-6 pre-B ALL cells.Materials and Methods: In an experimental study, to investigate the effect of BIBR1532, Nalm-6 leukemic cells were cultured in the presence of various concentrations of the inhibitor and consequently MTT assay, BrdU cell proliferation assay, flowcytometeric cell cycle analysis, and quantitative realtime PCR were applied.Results: Our results revealed that BIBR1532 induces an inhibitory effect on metabolic activity and DNA synthesis rate of Nalm-6 cells in a dose- and time-dependent manner. Moreover, we found that BIBR1532 exerts an inductive effect on mRNA expression level of Bax, Bad, p73, and p21, which in turn leads to G1 cell cycle arrest and increased sub-G1 cell population.Conclusions: Since treatment with BIBR1532 could arrest the cell cycle activity in Nalm-6 cells and activate cellular apoptotic pathway, anti-telomerase-based therapy may be regarded as a novel promising strategy for ALL treatment.

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APA: Copy

ZAREII, M., & BASHASH, D.. (2017). INDUCTION OF G₁ CELL CYCLE ARREST AND INCREASED SUB-G₁ POPULATION UPON TREATMENT OF NALM-6 CELLS WITH SYNTHETIC INHIBITOR OF HTERT. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 13(4), 314-323. SID. https://sid.ir/paper/78370/en

Vancouver: Copy

ZAREII M., BASHASH D.. INDUCTION OF G₁ CELL CYCLE ARREST AND INCREASED SUB-G₁ POPULATION UPON TREATMENT OF NALM-6 CELLS WITH SYNTHETIC INHIBITOR OF HTERT. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2017;13(4):314-323. Available from: https://sid.ir/paper/78370/en

IEEE: Copy

M. ZAREII, and D. BASHASH, “INDUCTION OF G₁ CELL CYCLE ARREST AND INCREASED SUB-G₁ POPULATION UPON TREATMENT OF NALM-6 CELLS WITH SYNTHETIC INHIBITOR OF HTERT,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 13, no. 4, pp. 314–323, 2017, [Online]. Available: https://sid.ir/paper/78370/en

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