PREVALENCE OF HEPATITIS B VIRUS GENOTYPES TO BE DETERMINED BY SEQUENCING IN BLOOD DONORS IN KERMAN, ESFAHAN AND YAZD

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SHARIFI ZOHREH; GHARAH BAGHIAN AHMAD; NOUROUZI MOZHGAN

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2010 | Volume:6 | Issue:4 (25) Page(s): 248-256

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Title

PREVALENCE OF HEPATITIS B VIRUS GENOTYPES TO BE DETERMINED BY SEQUENCING IN BLOOD DONORS IN KERMAN, ESFAHAN AND YAZD

Author(s)

SHARIFI ZOHREH | GHARAH BAGHIAN AHMAD | NOUROUZI MOZHGAN | Issue Writer Certificate

Keywords

HEPATITIS B VIRUSQ3

BLOOD DONORSQ2

GENOTYPEQ2

Abstract

Background and Objectives: Hepatitis B is one of the major diseases that have the potential to be transmitted by blood transfusion. Specific GENOTYPEs of HEPATITIS B VIRUS are increasingly recognized for their clinical significance and association with particular viral mutations. The aim of this study was to investigate the prevalence of HEPATITIS B VIRUS GENOTYPEs in BLOOD DONORS of Kerman, Yazd and Isfehan Blood Centers.Materials and Methods: Sera of 120 BLOOD DONORS, who were positive for hepatitis B surface antigen (HBsAg) using ELISA method, were selected. HBV DNA was extracted from sera using commercial kits and the p gene sequences were amplified by nested-PCR. HBV GENOTYPEs were determined by direct sequencing of the polymerase gene of HBV. The obtained sequences were aligned with Clustal W programme. Phylogenetic trees were constructed by the Neighbor-joining (NJ) method. The data were analyzed with Fisher test by SPSS 11.5.Results: Out of 120 HBsAg positive volunteer BLOOD DONORS, 69 (57.5%, CI 95%=48.65-66.34) were positive for HBV DNA sequence. Sequence analysis was done on the positive samples.Genotype D was detected among 100% of HBV infected volunteer BLOOD DONORS.Conclusions: The prevalence rate of GENOTYPE D was found to be 100%. These findings may have an impact on the immunological and genetic diagnosis of HBV as well as on the selection of diagnostic kits and viral quality control panels.

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APA: Copy

SHARIFI, ZOHREH, GHARAH BAGHIAN, AHMAD, & NOUROUZI, MOZHGAN. (2010). PREVALENCE OF HEPATITIS B VIRUS GENOTYPES TO BE DETERMINED BY SEQUENCING IN BLOOD DONORS IN KERMAN, ESFAHAN AND YAZD. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 6(4 (25)), 248-256. SID. https://sid.ir/paper/78397/en

Vancouver: Copy

SHARIFI ZOHREH, GHARAH BAGHIAN AHMAD, NOUROUZI MOZHGAN. PREVALENCE OF HEPATITIS B VIRUS GENOTYPES TO BE DETERMINED BY SEQUENCING IN BLOOD DONORS IN KERMAN, ESFAHAN AND YAZD. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2010;6(4 (25)):248-256. Available from: https://sid.ir/paper/78397/en

IEEE: Copy

ZOHREH SHARIFI, AHMAD GHARAH BAGHIAN, and MOZHGAN NOUROUZI, “PREVALENCE OF HEPATITIS B VIRUS GENOTYPES TO BE DETERMINED BY SEQUENCING IN BLOOD DONORS IN KERMAN, ESFAHAN AND YAZD,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 6, no. 4 (25), pp. 248–256, 2010, [Online]. Available: https://sid.ir/paper/78397/en

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