DETERMINATION OF
A/B 
RATIO IN HEALTHY INDIVIDUALS (18-45 YEARS OLD( 
WITH NORMAL HEMATOLOGICAL INDICES (MCV³80, 
MCH³27) 
AND NORMAL HBA2 BY CATION EXCHANGE CHROMATOGRAPHY

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HOSSEINI GOUHARI L.; NOORMOHAMMADI I.; SHARAFI TAFRESHI MOGHADAM A.A.; MOSTAAN L.; DROUSIOTOU A.

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Journal Paper

Paper Information

Journal: The Scientific Journal of Iranian Blood Transfusion Year:2007 | Volume:3 | Issue:4 Page(s): 325-331

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Title

DETERMINATION OF A/B RATIO IN HEALTHY INDIVIDUALS (18-45 YEARS OLD( WITH NORMAL HEMATOLOGICAL INDICES (MCV³80, MCH³27) AND NORMAL HBA2 BY CATION EXCHANGE CHROMATOGRAPHY

Author(s)

Keywords

Α GLOBINQ4

Β GLOBINQ4

HEMOGLOBINQ4

NORMAL RANGEQ4

CATION EXCHANGE CHROMATOGRAPHYQ4

Abstract

Background and Objectives: For a successful prevention program, globin chain synthesis, as a complementary test beside DNA analysis, is necessary. So, it is important that each laboratory establishes its own reference range for the classification of thalassemia syndromes by globin chain synthesis. Globin chain synthesis is a relatively complex test introduced in the study of thalassemia syndromes as a reference method. The technique is also useful for variant chain identification. This study aims to stablish the method of globin chain synthesis to determine a/b chain ratio in healthy individuals.Materials and Methods: In this study globin chain analysis was performed on 30 healthy laboratory personnel's with normal HbA2 and normal hematological indices. In this method a reticulocyte-rich sample is incubated with a mixture of amino acids, one of which (leucine) radioactively labeled. After washing the excess radioactivity and precipitating the globin, different chains are separated by CATION EXCHANGE CHROMATOGRAPHY.Results: The mean a/b ratio was 1.045±0.12 (mean±1SD) in healthy subjects. Our findings were in agreement with those of the other investigators in the world.Conclusions: In any screening program, diagnostic problems will arise that can not be solved without biosynthetic studies. The Clegg and Weather all method has been proved to be very reliable and reproducible, but time-consuming (requiring four days). New methods like reverse phase HPLC are now available for chain separation. Therefore, according to the procedures each laboratory should determine its own reference range.

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APA: Copy

HOSSEINI GOUHARI, L., NOORMOHAMMADI, I., SHARAFI TAFRESHI MOGHADAM, A.A., MOSTAAN, L., & DROUSIOTOU, A.. (2007). DETERMINATION OF A/B RATIO IN HEALTHY INDIVIDUALS (18-45 YEARS OLD( WITH NORMAL HEMATOLOGICAL INDICES (MCV³80, MCH³27) AND NORMAL HBA₂ BY CATION EXCHANGE CHROMATOGRAPHY. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 3(4), 325-331. SID. https://sid.ir/paper/78400/en

Vancouver: Copy

HOSSEINI GOUHARI L., NOORMOHAMMADI I., SHARAFI TAFRESHI MOGHADAM A.A., MOSTAAN L., DROUSIOTOU A.. DETERMINATION OF A/B RATIO IN HEALTHY INDIVIDUALS (18-45 YEARS OLD( WITH NORMAL HEMATOLOGICAL INDICES (MCV³80, MCH³27) AND NORMAL HBA₂ BY CATION EXCHANGE CHROMATOGRAPHY. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2007;3(4):325-331. Available from: https://sid.ir/paper/78400/en

IEEE: Copy

L. HOSSEINI GOUHARI, I. NOORMOHAMMADI, A.A. SHARAFI TAFRESHI MOGHADAM, L. MOSTAAN, and A. DROUSIOTOU, “DETERMINATION OF A/B RATIO IN HEALTHY INDIVIDUALS (18-45 YEARS OLD( WITH NORMAL HEMATOLOGICAL INDICES (MCV³80, MCH³27) AND NORMAL HBA₂ BY CATION EXCHANGE CHROMATOGRAPHY,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 3, no. 4, pp. 325–331, 2007, [Online]. Available: https://sid.ir/paper/78400/en

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