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Information Journal Paper

Title

Colonization of Mouse Spermatogonial Cells in Modified Soft Agar Culture System Utilizing Nanofibrous Scaffold: A New Approach

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  0-0

Abstract

 Background: Spermatogonial stem cells (SSCs) are considered in fertility management approaches of prepubertal boys facing cancer therapies. However, in vitro propagation has become an important issue due to a small number of SSCs in testicular tissue. The present study aimed to investigate a modified soft Agar culture system by using a nanofibrous scaffold as a new approach to mimic in vivo conditions of SSCs development. Materials and Methods: The SSCs were isolated from neonate mouse testes, cultured on polycaprolactone scaffold, and covered by a layer of soft Agar for 2 weeks. Then, the number and diameter of colonies formed in experimental groups were measured and spermatogonial markers (i. e., Plzf, Gfrα 1, Id4, and c-Kit) in SSCs colonies were evaluated by a real-time polymerase chain reaction and immunostaining. Results: Our results indicated that the colonization rate of SSCs was significantly higher in the present modified soft Agar culture system (P<0. 05). Only Plzf indicated a significant increased at the levels (P<0. 05), the gene expression levels of Id4, Plzf, and Gfrα 1 were higher in the present culture system. In addition, the expression of the c-Kit gene as a differentiating spermatogonia marker was higher in presence of scaffold and soft Agar compared with the amount of other experimental groups (P<0. 05). Conclusion: The culture system by using nanofibrous scaffold and soft Agar as a new culture method suggests the potential of this approach in SSCs enrichment and differentiation strategies for male infertility treatments, as well as in vitro spermatogenesis.

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    APA: Copy

    TALEBI, ALI, SADIGHI GILANI, MOHAMMAD ALI, KORUJI, MORTEZA, AI, JAFAR, REZAIE, MOHAMMAD JAFAR, NAVID, SHADAN, SALEHI, MAJID, & ABBASI, MEHDI. (2019). Colonization of Mouse Spermatogonial Cells in Modified Soft Agar Culture System Utilizing Nanofibrous Scaffold: A New Approach. GALEN MEDICAL JOURNAL, 8(1), 0-0. SID. https://sid.ir/paper/784463/en

    Vancouver: Copy

    TALEBI ALI, SADIGHI GILANI MOHAMMAD ALI, KORUJI MORTEZA, AI JAFAR, REZAIE MOHAMMAD JAFAR, NAVID SHADAN, SALEHI MAJID, ABBASI MEHDI. Colonization of Mouse Spermatogonial Cells in Modified Soft Agar Culture System Utilizing Nanofibrous Scaffold: A New Approach. GALEN MEDICAL JOURNAL[Internet]. 2019;8(1):0-0. Available from: https://sid.ir/paper/784463/en

    IEEE: Copy

    ALI TALEBI, MOHAMMAD ALI SADIGHI GILANI, MORTEZA KORUJI, JAFAR AI, MOHAMMAD JAFAR REZAIE, SHADAN NAVID, MAJID SALEHI, and MEHDI ABBASI, “Colonization of Mouse Spermatogonial Cells in Modified Soft Agar Culture System Utilizing Nanofibrous Scaffold: A New Approach,” GALEN MEDICAL JOURNAL, vol. 8, no. 1, pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/784463/en

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