SUBCUTANEOUS TRANSPLANTATION OF MARROW– DERIVED MURINE MESENCHYMAL STEM CELLS CULTIVATED IN ALGINATE AND THEIR CHONDROGENESIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ESLAMINEZHAD M.R.; TAGHIYAR LEYLA; KIANI SAHAR; PIRIAEI A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2007 | Volume:4 | Issue:2 Page(s): 105-114

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

962

Download:

Cites:

Information Journal Paper

Title

SUBCUTANEOUS TRANSPLANTATION OF MARROW– DERIVED MURINE MESENCHYMAL STEM CELLS CULTIVATED IN ALGINATE AND THEIR CHONDROGENESIS

Author(s)

ESLAMINEZHAD M.R. | TAGHIYAR LEYLA | KIANI SAHAR | PIRIAEI A. | Issue Writer Certificate

Keywords

MESENCHYMAL STEM CELLSQ2

ALGINATEQ4

TRANSPLANTATIONQ2

Abstract

Background and Objectives: MESENCHYMAL STEM CELLS are appropriate candidates to treat diseases including articular cartilage defects. There are plenty of researches being conducted to make the application of these cells possible. The purpose of this study was to cultivate murine MESENCHYMAL STEM CELLS (MSCs) in ALGINATE gel and transplant them subcutaneously to immuno-suppressed rats to examine their chondrogenic potential in vivo.Materials and Methods: 4-6 week old NMRI mice were sacrificed and their bone marrow cells were cultivated in 6-cell plates at the density of 500 cell/cm2. The pure fibroblastic cells appeared after two passages. 2×106 fibroblastic cells were mixed with 1 ml of ALGINATE solution and converted into gel by being exposed to calcium chloride solution. MSCs-embedded ALGINATE gel was then transplanted subcutaneously to 6 rats that had received an immunosuppressive drug (cyclosporine) for transplant rejection to be avoided. 5 weeks after TRANSPLANTATION, the ALGINATE gels were removed and evaluated by histochemistry, RT-PCR for certain cartilage markers, and transmission electron microscopy.Results: 5 weeks after TRANSPLANTATION, the skin was incised and the ALGINATE gel with its surrounding vascular connective tissue was removed. Tuloidine blue staining indicates that the cells within the gel assumed oval morphology and occupied lacuna-like cavities. RT-PCR analysis revealed that in these cells the mRNA of some cartilage markers such as collagen II (the marker of hyaline cartilage), collagen X (hypertrophied chondrocyte marker in osteogenesis), and aggreacan were largely produced. Ultra-thin sections analysis showed that the cells within the lacuna-like cavity of ALGINATE gel contain a large amount of expanded rough endoplasmic reticulum and secret fibrillar extra cellular matrix.Conclusions: Transplanted murine MSCs cultivated in ALGINATE gel can differentiate into hyaline cartilage with the sign of osteogenic initiation.

Cites

No record.

References

ISOLATION AND IN VITRO PROLIFERATION OF MESENCHYMAL STEM CELLS FROM NMRI MOUSE BONE MARROW

Cite

APA: Copy

ESLAMINEZHAD, M.R., TAGHIYAR, LEYLA, KIANI, SAHAR, & PIRIAEI, A.. (2007). SUBCUTANEOUS TRANSPLANTATION OF MARROW– DERIVED MURINE MESENCHYMAL STEM CELLS CULTIVATED IN ALGINATE AND THEIR CHONDROGENESIS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 4(2), 105-114. SID. https://sid.ir/paper/78456/en

Vancouver: Copy

ESLAMINEZHAD M.R., TAGHIYAR LEYLA, KIANI SAHAR, PIRIAEI A.. SUBCUTANEOUS TRANSPLANTATION OF MARROW– DERIVED MURINE MESENCHYMAL STEM CELLS CULTIVATED IN ALGINATE AND THEIR CHONDROGENESIS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2007;4(2):105-114. Available from: https://sid.ir/paper/78456/en

IEEE: Copy

M.R. ESLAMINEZHAD, LEYLA TAGHIYAR, SAHAR KIANI, and A. PIRIAEI, “SUBCUTANEOUS TRANSPLANTATION OF MARROW– DERIVED MURINE MESENCHYMAL STEM CELLS CULTIVATED IN ALGINATE AND THEIR CHONDROGENESIS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 4, no. 2, pp. 105–114, 2007, [Online]. Available: https://sid.ir/paper/78456/en

Related Journal Papers