PHYLOGENETIC ANALYSIS OF HIV-1 POL GENE (RT SEQUENCES) AMONG IRANIAN HIV INFECTED PATIENTS

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BAESI K.; RAVANSHAD M.; HOSSEINI S.Y.; HAJI ABD ALBAGHI MAHBOUBEH; SHAHZAMANI K.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2010 | Volume:7 | Issue:2 (27) Page(s): 94-100

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Title

PHYLOGENETIC ANALYSIS OF HIV-1 POL GENE (RT SEQUENCES) AMONG IRANIAN HIV INFECTED PATIENTS

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Keywords

HIV-1Q3

IRANQ3

GENETIC DIVERSITYQ3

Abstract

Background and Objectives: Human immunodeficiency virus (HIV) is the retrovirus that causes the disease known as AIDS. In the current study, we examined the GENETIC DIVERSITY of HIV-1 strains circulating in IRAN.Materials and Methods: Sequencing and phylogenetic analysis of human immunodeficiency virus type 1 (HIV-1) pol gene is a useful method for subtyping strains of HIV-1. HIV-1 RNA was derived from plasma of 24 infected patients in Imam Khomeini Hospital in Tehran. RT Nested-PCR was developed and the product was extracted from agarose gel. The products were sequenced and phylogenetic analysis by Mega 4 software was constructed.Results: The developed PCR method successfully amplified the specific sequence. The sequence and phylogenetic analysis showed subtypes A1 and B in 14 and 10 patients, respectively.Conclusions: The results indicated intravenous drug users infected with HIV-1 subtype A1, and hemophiliacs infected with HIV-1 subtype B. The results also showed that the subtype A1 is the most prevalent in HIV infected patients. Such information can be useful in intervention strategies and control programs.

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APA: Copy

BAESI, K., RAVANSHAD, M., HOSSEINI, S.Y., HAJI ABD ALBAGHI, MAHBOUBEH, & SHAHZAMANI, K.. (2010). PHYLOGENETIC ANALYSIS OF HIV-1 POL GENE (RT SEQUENCES) AMONG IRANIAN HIV INFECTED PATIENTS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 7(2 (27)), 94-100. SID. https://sid.ir/paper/78473/en

Vancouver: Copy

BAESI K., RAVANSHAD M., HOSSEINI S.Y., HAJI ABD ALBAGHI MAHBOUBEH, SHAHZAMANI K.. PHYLOGENETIC ANALYSIS OF HIV-1 POL GENE (RT SEQUENCES) AMONG IRANIAN HIV INFECTED PATIENTS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2010;7(2 (27)):94-100. Available from: https://sid.ir/paper/78473/en

IEEE: Copy

K. BAESI, M. RAVANSHAD, S.Y. HOSSEINI, MAHBOUBEH HAJI ABD ALBAGHI, and K. SHAHZAMANI, “PHYLOGENETIC ANALYSIS OF HIV-1 POL GENE (RT SEQUENCES) AMONG IRANIAN HIV INFECTED PATIENTS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 7, no. 2 (27), pp. 94–100, 2010, [Online]. Available: https://sid.ir/paper/78473/en

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