Characterization of the decellularized ovine pericardium for skin tissue engineering

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ALIZADEH MORTEZA; Rezakhani Leila; KHODAEI MOHAMMAD; ALIZADEH AKRAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahrekord University of Medical Sciences Year:2020 | Volume:22 | Issue:4 Page(s): 173-180

Download Full-Text

View:

145

Download:

Cites:

Information Journal Paper

Title

Characterization of the decellularized ovine pericardium for skin tissue engineering

Author(s)

ALIZADEH MORTEZA | Rezakhani Leila | KHODAEI MOHAMMAD | ALIZADEH AKRAM | Issue Writer Certificate

Keywords

Ovine pericardium

Acellular

Skin tissue engineering

Abstract

Background and aims: Some biological scaffolds are used as appropriate skin substitutes, including decellularized amniotic membrane or dermis although the ovine pericardial tissue has not been characterized or used for Skin tissue engineering. In this regard, this study focused on the decellularization and characterization of Ovine pericardium for Skin tissue engineering. Materials and Methods: To this end, two different methods were used for decellularization, including safety data sheet (SDS) 1% (method 1) and Triton X-100 1% (method 2). In addition, histological examinations (H&E staining), DNA content assay, scanning electron microscopy (SEM), MTT test using human adipose-derived mesenchymal stem cells, and tensile tests were conducted for sample characterization. Results: Based on the results, the DNA content showed significant DNA removing (P < 0. 001) after decellularization with methods 1 and 2 in comparison to native tissues although the significance level between the two methods was P = 0. 06. In the SEM examination, cells were effectively removed while the extrAcellular matrix remained intact in both groups. Based on the results of the MTT test, the toxicity was not significant (P = 0. 36). On the other hand, mechanical property assay revealed a higher value of Young’ s modulus in method 1 (34. 12 MPa) compared to Method 2 (32. 57 MPa) and native tissues (30 MPa). Finally, the highest strain at the break point (approximately 0. 6) belonged to the native sample. Conclusion: In general, the ovine seems to be a good alternative for Skin tissue engineering and regeneration since it is a post-slaughtering waste tissue has low thickness, is wide and spread, and easy to be decellularized with SDS 1% and Triton X-100. Eventually, it has good properties for cell seeding based on the findings of our study and the capability of vascularization reported in the literature.

Cites

No record.

References

Cite

APA: Copy

ALIZADEH, MORTEZA, Rezakhani, Leila, KHODAEI, MOHAMMAD, & ALIZADEH, AKRAM. (2020). Characterization of the decellularized ovine pericardium for skin tissue engineering. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 22(4), 173-180. SID. https://sid.ir/paper/784798/en

Vancouver: Copy

ALIZADEH MORTEZA, Rezakhani Leila, KHODAEI MOHAMMAD, ALIZADEH AKRAM. Characterization of the decellularized ovine pericardium for skin tissue engineering. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;22(4):173-180. Available from: https://sid.ir/paper/784798/en

IEEE: Copy

MORTEZA ALIZADEH, Leila Rezakhani, MOHAMMAD KHODAEI, and AKRAM ALIZADEH, “Characterization of the decellularized ovine pericardium for skin tissue engineering,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 4, pp. 173–180, 2020, [Online]. Available: https://sid.ir/paper/784798/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops