INFECTION OF MSCS BY ADENOVIRUS EXPRESSION SYSTEM EXPRESSING NRF2 AS A CYTOPROTECTIVE FACTOR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHAMMADZADEH M.; HALABIAN R.; MOHAMMADIPOOR M.; KIANI A.A.; GHAREHBAGHIAN A.; AMIRIZADEH N.; HABIBI ROUDKENAR M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2012 | Volume:8 | Issue:4 (33) Page(s): 272-285

Download Full-Text

Persian Verion

View:

1,238

Download:

Cites:

Information Journal Paper

Title

INFECTION OF MSCS BY ADENOVIRUS EXPRESSION SYSTEM EXPRESSING NRF2 AS A CYTOPROTECTIVE FACTOR

Author(s)

Keywords

MESENCHYMAL STEM CELLSQ3

NRF2 PROTEINQ3

TOPOQ3

Abstract

Background and Objectives: Poor viability of MESENCHYMAL STEM CELLS (MSCs) following transplantation is one of the major challenges in their therapeutic application. Manipulation of MSCs by the genetic engineering method is one of the strategies used to protect the cells against cytotoxic microenvironment. However, maintaining multi differentiation capacity of MSCs following manipulation is important. We investigated if the manipulation of MSCs with NRF2 affects the multi differentiation capacity.Materials and Methods: MSCs were isolated from bone marrow. NRF2 was isolated and TOPO cloned into the pENTR vector. The recombinant vector was transferred into pAD/CMV/V5-DEST vector by gateway technology. Recombinant adenovirus was produced in AD293 cells, followed by being infected into MSCs. Expression of NRF2 was verified by RT-PCR. The NRF2 engineered MSCs were exposed to stress conditions followed by the evaluation of the cells viability and apoptosis. Finally, NRF2 expressing MSCs differentiation into osteoblast and adipocyte lineages was studied.Results: NRF2 was successfully expressed in MSCs. NRF2-MSCs differentiation into osteoblast and adipocyte lineages indicating overexpression of NRF2 does not affect the differentiation property of MSCs.Conclusions: Expression of NRF2, a well known cytoprotective factor, by using adenovirus expression system does not intervene in the differentiation capacity of MSCs. NRF2-MSCs might be applicable for stem cell-based cell therapy in future.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHAMMADZADEH, M., HALABIAN, R., MOHAMMADIPOOR, M., KIANI, A.A., GHAREHBAGHIAN, A., AMIRIZADEH, N., & HABIBI ROUDKENAR, M.. (2012). INFECTION OF MSCS BY ADENOVIRUS EXPRESSION SYSTEM EXPRESSING NRF2 AS A CYTOPROTECTIVE FACTOR. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 8(4 (33)), 272-285. SID. https://sid.ir/paper/78521/en

Vancouver: Copy

MOHAMMADZADEH M., HALABIAN R., MOHAMMADIPOOR M., KIANI A.A., GHAREHBAGHIAN A., AMIRIZADEH N., HABIBI ROUDKENAR M.. INFECTION OF MSCS BY ADENOVIRUS EXPRESSION SYSTEM EXPRESSING NRF2 AS A CYTOPROTECTIVE FACTOR. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2012;8(4 (33)):272-285. Available from: https://sid.ir/paper/78521/en

IEEE: Copy

M. MOHAMMADZADEH, R. HALABIAN, M. MOHAMMADIPOOR, A.A. KIANI, A. GHAREHBAGHIAN, N. AMIRIZADEH, and M. HABIBI ROUDKENAR, “INFECTION OF MSCS BY ADENOVIRUS EXPRESSION SYSTEM EXPRESSING NRF2 AS A CYTOPROTECTIVE FACTOR,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 8, no. 4 (33), pp. 272–285, 2012, [Online]. Available: https://sid.ir/paper/78521/en

Related Journal Papers