PREPARATION OF HEME CONTROL FOR CELLULOSE ACETATE ELECTROPHORESIS

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HOSSEINI GOUHARI L.; NOORMOHAMMADI E.; ATAR CHI Z.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2005 | Volume:2 | Issue:3 Page(s): 53-58

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Title

PREPARATION OF HEME CONTROL FOR CELLULOSE ACETATE ELECTROPHORESIS

Author(s)

HOSSEINI GOUHARI L. | NOORMOHAMMADI E. | ATAR CHI Z. | Issue Writer Certificate

Keywords

HEMOGLOBINQ3

CELLULOSE ACETATEQ4

ELECTROPHORESISQ4

HEMOGLOBINOPATHYQ4

Abstract

Background and Objectives To avoid laboratory errors in the detection of hemoglobinopathies, a control sample containing HEMOGLOBIN (Hb) A,F,S or C should run with each set of samples.The aim of this project was to prepare a lyophilized heme control for the CELLULOSE ACETATE ELECTROPHORESIS.Materials and MethodsAfter lysing the red blood cells, the hemolysate was centrifuged at 25000 rpm. The clear red supernatant was diluted with drabkin reagent to 20-30 gr/L and Hb was converted to cyanomethemoglobin. After adding sucrose and preservatives, the hemolysate was passed through milipore filter (0.45 µm) and then aliqouted and lyophilized.ResultsIn this project three heme control samples were prepared. The first sample contains Hbs A,F,D or G ,the second one contains Hbs A,S, and the third contains Hb A,F. Although the amount of potassium cyanide used is 10 times less than other methods , the stability is more than 6 months at 4oC; moreover, and the electrophoretic patterns have good resolution and the obtained CV and SD show good reproducibility.ConclusionsThe preparation method in this project is simple, reliable, cost effective and in comparison with other methods has less amount of potassium cyanide; therefore, it is safe for the laboratory staff.

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APA: Copy

HOSSEINI GOUHARI, L., NOORMOHAMMADI, E., & ATAR CHI, Z.. (2005). PREPARATION OF HEME CONTROL FOR CELLULOSE ACETATE ELECTROPHORESIS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 2(3), 53-58. SID. https://sid.ir/paper/78562/en

Vancouver: Copy

HOSSEINI GOUHARI L., NOORMOHAMMADI E., ATAR CHI Z.. PREPARATION OF HEME CONTROL FOR CELLULOSE ACETATE ELECTROPHORESIS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2005;2(3):53-58. Available from: https://sid.ir/paper/78562/en

IEEE: Copy

L. HOSSEINI GOUHARI, E. NOORMOHAMMADI, and Z. ATAR CHI, “PREPARATION OF HEME CONTROL FOR CELLULOSE ACETATE ELECTROPHORESIS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 2, no. 3, pp. 53–58, 2005, [Online]. Available: https://sid.ir/paper/78562/en

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