EVALUATION OF EXPANSION AND DIFFERENTIATION OF CORD BLOOD HEMATOPOIETIC CELLS EXPANDED IN SUSPENSION BIOREACTOR WITH VERTICAL AGITATION

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SHAYAN ASL N.; EBRAHIMI M.; BEIKI B.; JANZAMIN E.; BANI ARDALAN P.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2013 | Volume:10 | Issue:2 (39) Page(s): 112-121

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Title

EVALUATION OF EXPANSION AND DIFFERENTIATION OF CORD BLOOD HEMATOPOIETIC CELLS EXPANDED IN SUSPENSION BIOREACTOR WITH VERTICAL AGITATION

Author(s)

Keywords

CORD BLOODQ2

HEMATOPOIETIC STEM CELLSQ2

BIOREACTORSQ2

Abstract

Background and Objectives: Umblical CORD BLOOD (UCB) HEMATOPOIETIC STEM CELLS having high cell counts are suitable candidates for benign and malignant disease transplantation but often UCBs have low counts. In this study, ex vivo expansion of these cells was performed using suspension bioreactor with vertical agitation.Materials and Methods: UCB Mononuclear cells were cultured with 1.07´106 cells/ml density in total 206 ml of culture medium in the suspension bioreactor with vertical agitator; and the static group with same density was cultured in T-flasks for 14 days. At the days 0, 3, 7 and 14 of culture, sampling was performed for further analysis.Results: During the 3 days of culture, differentiated cells were lost and then the number of cells increased again from 0.42´106 cells/ml up to 1.2´106 cells/ml and from 0.6´106 up to 2.9´106 in bioreactor and static culture, respectively. The percentage of CD34+ cells and colony forming potential significantly increased in bioreactor.Conclusions: HEMATOPOIETIC STEM CELLS in the bioreactor while retaining their self-potential are expanded. However, due to the increasing shear stress in the environment, the total number of cells did not increase. Therefore, further studies are required.

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APA: Copy

SHAYAN ASL, N., EBRAHIMI, M., BEIKI, B., JANZAMIN, E., & BANI ARDALAN, P.. (2013). EVALUATION OF EXPANSION AND DIFFERENTIATION OF CORD BLOOD HEMATOPOIETIC CELLS EXPANDED IN SUSPENSION BIOREACTOR WITH VERTICAL AGITATION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 10(2 (39)), 112-121. SID. https://sid.ir/paper/78595/en

Vancouver: Copy

SHAYAN ASL N., EBRAHIMI M., BEIKI B., JANZAMIN E., BANI ARDALAN P.. EVALUATION OF EXPANSION AND DIFFERENTIATION OF CORD BLOOD HEMATOPOIETIC CELLS EXPANDED IN SUSPENSION BIOREACTOR WITH VERTICAL AGITATION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2013;10(2 (39)):112-121. Available from: https://sid.ir/paper/78595/en

IEEE: Copy

N. SHAYAN ASL, M. EBRAHIMI, B. BEIKI, E. JANZAMIN, and P. BANI ARDALAN, “EVALUATION OF EXPANSION AND DIFFERENTIATION OF CORD BLOOD HEMATOPOIETIC CELLS EXPANDED IN SUSPENSION BIOREACTOR WITH VERTICAL AGITATION,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 10, no. 2 (39), pp. 112–121, 2013, [Online]. Available: https://sid.ir/paper/78595/en

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