EVALUATION OF BACTERIAL CONTAMINATION OF PLATELET CONCENTRATES COLLECTED AT TEHRAN REGIONAL BLOOD CENTER

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AHMADI JAHANGIR; GHOLIZADEH H.R.; FARSEH R.; SHARIFI SH.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2006 | Volume:2 | Issue:6 Page(s): 233-236

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Title

EVALUATION OF BACTERIAL CONTAMINATION OF PLATELET CONCENTRATES COLLECTED AT TEHRAN REGIONAL BLOOD CENTER

Author(s)

AHMADI JAHANGIR | GHOLIZADEH H.R. | FARSEH R. | SHARIFI SH. | Issue Writer Certificate

Keywords

BLOOD TRANSFUSIONQ2

PLATELET CONCENTRATESQ4

BACTERIAL CONTAMINATIONQ4

Abstract

In spite of major advances in the field of quality assurance in the process of collection, preparation and storage of platelets, bacterial infection following platelet transfusion remains a major problem in transfusion medicine. The present study was carried out in order to evaluate BACTERIAL CONTAMINATION of PLATELET CONCENTRATES collected at Tehran Regional Educational BLOOD TRANSFUSION Center. Materials and Methods Bacterial growth of samples of PLATELET CONCENTRATES was studied in blood agar, EMB and thioglycollate broth after 48 hours at 37ºC. The use of differentiation tests was made when any bacterial growth was observed. Simultaneously, the samples were also cultured in thioglycollate broth and studied for any turbidity or color change within 7 days. Any changes made the samples to be cultured in blood agar and EMB. Finally, the contamination rate and the ratio of contaminating bacteria were determined. Out of 7700 samples, three fourth (5775 samples) were taken from the cord and one fourth (1925) from both the bag and the cord. Results Out of 7700 samples of PLATELET CONCENTRATES studied, 14 (0.18%) were found positive for BACTERIAL CONTAMINATION. The contamination rate was estimated to be one in every 550 tested platelets (0.18%). Since in cases of blood bag contamination, the cord had been contaminated as well, there was then no difference on whether the sample was taken from the bag or cord. The bacteria identified were as follows: Staph. epidermidis (n=4), Staph. saprophyticus (n=2), Acinetobacter (n=5), Bacillus sp. (n=3). Conclusions The results show that screening PLATELET CONCENTRATES for BACTERIAL CONTAMINATION is necessary for BLOOD TRANSFUSION centers and hospital blood banks.

Cites

EVALUATION OF THE EFFECT OF VIRKON DISINFECTANT IN REDUCING BACTERIAL CONTAMINATION OF PLATELET COMPONENTS

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APA: Copy

AHMADI, JAHANGIR, GHOLIZADEH, H.R., FARSEH, R., & SHARIFI, SH.. (2006). EVALUATION OF BACTERIAL CONTAMINATION OF PLATELET CONCENTRATES COLLECTED AT TEHRAN REGIONAL BLOOD CENTER. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 2(6), 233-236. SID. https://sid.ir/paper/78614/en

Vancouver: Copy

AHMADI JAHANGIR, GHOLIZADEH H.R., FARSEH R., SHARIFI SH.. EVALUATION OF BACTERIAL CONTAMINATION OF PLATELET CONCENTRATES COLLECTED AT TEHRAN REGIONAL BLOOD CENTER. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2006;2(6):233-236. Available from: https://sid.ir/paper/78614/en

IEEE: Copy

JAHANGIR AHMADI, H.R. GHOLIZADEH, R. FARSEH, and SH. SHARIFI, “EVALUATION OF BACTERIAL CONTAMINATION OF PLATELET CONCENTRATES COLLECTED AT TEHRAN REGIONAL BLOOD CENTER,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 2, no. 6, pp. 233–236, 2006, [Online]. Available: https://sid.ir/paper/78614/en

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