THE PATTERN OF CROSS LINEAGE T-CELL RECEPTOR Δ/Γ GENE REARRANGEMENTS IN B-PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA OF IRANIAN CHILDREN USING POLYMERASE CHAIN REACTION

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POUPAK B.; POURFATH ELAH A.A.; NAJMABAD H.; MORTAZAVI YOUSEF; YAHYAVI S.H.; HAGHNEZHAD DOUSHANLOU F.; VOSOUGH PARVANEH; ARZANIAN M.T.; IZADYAR MINA; SHAHGHOLI E.; BAHOUSH GH.R.; HAMIDIEH A.A.; FARANOUSH M.; KHOSRAVIPOUR G.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2006 | Volume:2 | Issue:6 Page(s): 203-214

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Title

THE PATTERN OF CROSS LINEAGE T-CELL RECEPTOR Δ/Γ GENE REARRANGEMENTS IN B-PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA OF IRANIAN CHILDREN USING POLYMERASE CHAIN REACTION

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Keywords

GENE REARRANGEMENTQ4

CLONALITYQ4

MINIMAL RESIDUAL DISEASE (MRD)Q4

Abstract

Background and Objectives Diversity in heavy chain immunoglobulin (IgH) and T-cell receptor (TCR) molecules occurs during B- and T-lymphocyte differentiation through the rearrangement of variable (V), diversity (D), junction (J) and constant (C) gene segments. Lymphoid leukemia cells are similar to normal precursors and have rearranged IgH, Igк and TCR (cross-lineage rearrangement) genes which can be used as a marker of CLONALITY and evaluation of minimal residual disease (MRD). The purpose of this study is to evaluate the pattern of TCR- δ/γ – GENE REARRANGEMENTs using Polymerase Chain Reaction (PCR) in B-precursor acute lymphoblastic l eukemia (ALL) in Iranian children. Materials and Methods In our prospective study, bone marrow aspirates of 183 children with early diagnosis of acute leukemia were collected at admission before any chemotherapy. After reviewing cytomorphology and immunophenotyping, only 140 subjects with diagnosis of B-precursor ALLs were selected for study. Sixteen were excluded from our study due to various reasons including cellular degeneration. Mononuclear cells including leukemic blasts were isolated by density gradient. After DNA extraction, hyper-variable regions TCR-δ (Vδ2-Dδ3 and Dδ2- Dδ3 and TCR - γ (V γ ;V γ I V γ II) were amplified by consensus primers using PCR. PCR products were analyzed after heteroduplex analysis and polyacrylamide gel electrophoresis (silver stain). The DNA sequences were then compared and aligned to the homologous sequences of Gene Bank for confirmation. T-test, Mann whitney, Fisher exact test and Chi-square were used for data analysis. Results Clonal rearrangement of TCR -γ (V γ) and V γI/II were present in 79.3% and 64.9% of patients respectively and only 5% of cases showed biclonal pattern. The V γ II rearrangement was the most common (46.8%) type in TCR- γ. 47 (45.2%) and 11 (16.6%) of patients had Vδ2- Dδ3 and Dδ2– Dδ3partial GENE REARRANGEMENTs, respectively. Biclonal/oligoclonal patterns were present respectively in 27.7% and 4.3% of cases with Vδ2-Dδ3 rearrangement. Only one patient had biclonal Dδ2- Dδ3 rearrangement. Conclusions Clonal rearrangement of TCR-δ (Vδ2-Dδ3 and Dδ2- Dδ3) genes had a pattern similar to other populations. Frequency of TCR- γ (V γ I and V γ II) rearrangements was slightly higher than previous reports, and in contrary to others except for Brazilian report the V γII rearrangement was the most common type. We found no significant correlation between presence of different types of rearrangements and quantitative variables. The only significant point was the reduction of V+2D+3 with increase in age . According to preliminary results, these clonal markers can be used in diagnosis and follow up of MRD.

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APA: Copy

POUPAK, B., POURFATH ELAH, A.A., NAJMABAD, H., MORTAZAVI, YOUSEF, YAHYAVI, S.H., HAGHNEZHAD DOUSHANLOU, F., VOSOUGH, PARVANEH, ARZANIAN, M.T., IZADYAR, MINA, SHAHGHOLI, E., BAHOUSH, GH.R., HAMIDIEH, A.A., FARANOUSH, M., & KHOSRAVIPOUR, G.. (2006). THE PATTERN OF CROSS LINEAGE T-CELL RECEPTOR Δ/Γ GENE REARRANGEMENTS IN B-PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA OF IRANIAN CHILDREN USING POLYMERASE CHAIN REACTION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 2(6), 203-214. SID. https://sid.ir/paper/78618/en

Vancouver: Copy

POUPAK B., POURFATH ELAH A.A., NAJMABAD H., MORTAZAVI YOUSEF, YAHYAVI S.H., HAGHNEZHAD DOUSHANLOU F., VOSOUGH PARVANEH, ARZANIAN M.T., IZADYAR MINA, SHAHGHOLI E., BAHOUSH GH.R., HAMIDIEH A.A., FARANOUSH M., KHOSRAVIPOUR G.. THE PATTERN OF CROSS LINEAGE T-CELL RECEPTOR Δ/Γ GENE REARRANGEMENTS IN B-PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA OF IRANIAN CHILDREN USING POLYMERASE CHAIN REACTION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2006;2(6):203-214. Available from: https://sid.ir/paper/78618/en

IEEE: Copy

B. POUPAK, A.A. POURFATH ELAH, H. NAJMABAD, YOUSEF MORTAZAVI, S.H. YAHYAVI, F. HAGHNEZHAD DOUSHANLOU, PARVANEH VOSOUGH, M.T. ARZANIAN, MINA IZADYAR, E. SHAHGHOLI, GH.R. BAHOUSH, A.A. HAMIDIEH, M. FARANOUSH, and G. KHOSRAVIPOUR, “THE PATTERN OF CROSS LINEAGE T-CELL RECEPTOR Δ/Γ GENE REARRANGEMENTS IN B-PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA OF IRANIAN CHILDREN USING POLYMERASE CHAIN REACTION,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 2, no. 6, pp. 203–214, 2006, [Online]. Available: https://sid.ir/paper/78618/en

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