THE INHIBITORY EFFECTS OF CUMIN ALDEHYDE ON AMYLOID FIBRILLATION AND CYTOTOXICITY OF ALPHA-SYNUCLEIN

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MORSHEDI DINA; ALIAKBARI FARHANG

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Journal: Pathobiology Research Year:2012 | Volume:15 | Issue:1 Page(s): 45-60

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Title

THE INHIBITORY EFFECTS OF CUMIN ALDEHYDE ON AMYLOID FIBRILLATION AND CYTOTOXICITY OF ALPHA-SYNUCLEIN

Author(s)

MORSHEDI DINA | ALIAKBARI FARHANG | Issue Writer Certificate

Keywords

ALPHA-SYNUCLEINQ2

CUMIN ALDEHYDEQ2

FIBRIL INHIBITORQ2

PARKINSON'SQ2

Abstract

Objective: ALPHA-SYNUCLEIN is a major component of protein plaques in synucleinopathies, particularly Parkinson’s disease. The purpose of this study is to assess the inhibitory effects of CUMIN ALDEHYDE on the fibrillation of ALPHA-SYNUCLEIN. Methods: ALPHA-SYNUCLEIN was expressed in Escherichia coli and subsequently purified. For the process of fibrillation, purified protein was incubated at 37OC and pH 7.2. Fibrillation was analyzed by the standard fibril methods. The effects of different concentrations of CUMIN ALDEHYDE (20-500 mM) on ALPHA-SYNUCLEIN fibrillation were studied by assessment of the cytotoxic effects of samples on the neuroblastoma cell line, SK-N-MC. To study the protein aggregation forms that were generated in the presence of CUMIN ALDEHYDE, SDS resistance and induced fibrillation (seeding) methods were employed. For studying its specificity on ALPHA-SYNUCLEIN, the effect of CUMIN ALDEHYDE on lysozyme fibrillation was also examined.Results: We showed, for the first time, that CUMIN ALDEHYDE inhibited fibrillation by more than 80%. The highest inhibition was observed at the ratio of 5-15 moles of drug to protein. The viability of the treated cells with inhibited proteins was more than 90%, whereas non-inhibited samples caused a decrease in viability by 50%. Inhibited samples were not resistant to SDS and they were unable to induce fibrillation. CUMIN ALDEHYDE did not inhibit lysozyme fibrillation.Conclusion: CUMIN ALDEHYDE inhibited fibrillation of ALPHA-SYNUCLEIN which was accompanied by small amorphous aggregated particles of alpha synuclein. The inhibited protein samples did not induce aggregation. Thus, CUMIN ALDEHYDE can be considered as a candidate to inhibit the formation of ALPHA-SYNUCLEIN plaques.

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APA: Copy

MORSHEDI, DINA, & ALIAKBARI, FARHANG. (2012). THE INHIBITORY EFFECTS OF CUMIN ALDEHYDE ON AMYLOID FIBRILLATION AND CYTOTOXICITY OF ALPHA-SYNUCLEIN. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 15(1), 45-60. SID. https://sid.ir/paper/80981/en

Vancouver: Copy

MORSHEDI DINA, ALIAKBARI FARHANG. THE INHIBITORY EFFECTS OF CUMIN ALDEHYDE ON AMYLOID FIBRILLATION AND CYTOTOXICITY OF ALPHA-SYNUCLEIN. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2012;15(1):45-60. Available from: https://sid.ir/paper/80981/en

IEEE: Copy

DINA MORSHEDI, and FARHANG ALIAKBARI, “THE INHIBITORY EFFECTS OF CUMIN ALDEHYDE ON AMYLOID FIBRILLATION AND CYTOTOXICITY OF ALPHA-SYNUCLEIN,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 15, no. 1, pp. 45–60, 2012, [Online]. Available: https://sid.ir/paper/80981/en

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