A RAPID METHOD FOR EXTRACTION OF WATER SOLUBLE β (1,3) GLUCAN FROM THE CELL WALL OF CANDIDA ALBICANS

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NASROLLAHI ZAHRA; ROUDBAR MOHAMMADI SHAHLA; ATYABI FATEMEH; SARRAF ZUHAIR(HASSAN); YADEGARI MOHAMMAD HOSSEIN; ESFANDYARI MEHDI; MOLLARAZI ESMAIL

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Journal: Pathobiology Research Year:2013 | Volume:16 | Issue:1 Page(s): 90-97

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Title

A RAPID METHOD FOR EXTRACTION OF WATER SOLUBLE β (1,3) GLUCAN FROM THE CELL WALL OF CANDIDA ALBICANS

Author(s)

Keywords

β (1Q2

3) D-GLUCANSQ2

OXIDATION OF CELL WALL OF CANDIDA ALBICANSQ2

ENZYMATIC EXTRACTIONQ2

Abstract

Objective: In recent decades, b-glucans have been used as important complementary and alternative medicines for numerous immunocompromised individuals and those with advanced cancer. The most active form of b-glucans is b (1, 3) D-glucan and its most common source is cell wall of Candida albicans. Recently it has been introduced as a nano particle design to be used as a carrier for drug delivery. The current study researches a rapid method for the extraction of b (1, 3) D-glucans.Methods: The present study was conducted at Tarbiat Modares Medical University in 2012. Candida solubilized b-glucans were obtained by oxidation of the cell wall with sodium hypochlorite and sodium hydroxide. The particle part could be solubilized by treatment with dimethylsulfoxide (DMSO) and zymolyase digestion to extract b (1, 3)Dglucan. The soluble fractions were lyophilized. We performed the Callose test to verify the presence of b (1, 3) D-glucans. Solubilized fractions were dissolved in D2O and 1HNMR spectra were measured.Results: The soluble b (1, 3) D-glucan fraction which was derived from 1 g of dried Candida albicans germ tube weighed 190 mg. b (1, 3) D-glucan was verified by the Callose test and 1H-NMR test compared with Curdlan (standard). 1H-NMR spectra verified the existence of b (1, 3) D-glucan in the final product.Conclusion: In the present study, extraction of b (1, 3) D-glucan by oxidation of the cell wall using sodium hypochlorite yielded more pure b (1, 3) D-glucans in comparison with other extraction methods. Thus it might represent a rapid method of extraction.

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APA: Copy

NASROLLAHI, ZAHRA, ROUDBAR MOHAMMADI, SHAHLA, ATYABI, FATEMEH, SARRAF, ZUHAIR(HASSAN), YADEGARI, MOHAMMAD HOSSEIN, ESFANDYARI, MEHDI, & MOLLARAZI, ESMAIL. (2013). A RAPID METHOD FOR EXTRACTION OF WATER SOLUBLE β (1,3) GLUCAN FROM THE CELL WALL OF CANDIDA ALBICANS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 16(1), 90-97. SID. https://sid.ir/paper/81173/en

Vancouver: Copy

NASROLLAHI ZAHRA, ROUDBAR MOHAMMADI SHAHLA, ATYABI FATEMEH, SARRAF ZUHAIR(HASSAN), YADEGARI MOHAMMAD HOSSEIN, ESFANDYARI MEHDI, MOLLARAZI ESMAIL. A RAPID METHOD FOR EXTRACTION OF WATER SOLUBLE β (1,3) GLUCAN FROM THE CELL WALL OF CANDIDA ALBICANS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2013;16(1):90-97. Available from: https://sid.ir/paper/81173/en

IEEE: Copy

ZAHRA NASROLLAHI, SHAHLA ROUDBAR MOHAMMADI, FATEMEH ATYABI, ZUHAIR(HASSAN) SARRAF, MOHAMMAD HOSSEIN YADEGARI, MEHDI ESFANDYARI, and ESMAIL MOLLARAZI, “A RAPID METHOD FOR EXTRACTION OF WATER SOLUBLE β (1,3) GLUCAN FROM THE CELL WALL OF CANDIDA ALBICANS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 16, no. 1, pp. 90–97, 2013, [Online]. Available: https://sid.ir/paper/81173/en

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