PRODUCTION OF ANTIBODY AGAINST CTXB RECOMBINANT PROTEIN BASED ON NANOFIBERS AND NANOCAPSULES

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MASTI YOUSEF; SAADATI MOJTABA; NAZARIAN SHAHRAM; FASIHI RAMANDI MAHDI; ASADI MORTEZA; AREFPOOR MOHAMMADALI

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Journal: Pathobiology Research Year:2015 | Volume:17 | Issue:4 Page(s): 53-61

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Title

PRODUCTION OF ANTIBODY AGAINST CTXB RECOMBINANT PROTEIN BASED ON NANOFIBERS AND NANOCAPSULES

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Keywords

NANOFIBERQ2

NANOCAPSULEQ2

CHITOSANQ2

CTXBQ3

ANTIBODY TITERQ3

Abstract

CtxB (Cholera toxin B subunit) contributes to a vaccine's efficacy by stimulating production of the anti-CtxB antibody. Various attempts have been made to increase production of this antibody. CHITOSAN is a mucoadhesive polysaccharide that has tremendous potential for oral vaccine delivery in terms of its exclusive features that include biocompatibility, biodegradability, high charge density and non-toxicity. We investigated the potential for CHITOSAN NANOFIBERs and NANOCAPSULEs as novel carrier systems for the oral delivery of CTXB.Methods: Antigen-containing CHITOSAN NANOFIBERs were prepared by electrospinning a CHITOSAN/AcOH solution. Encapsulation of the antigen inside the CHITOSAN NANOFIBERs was confirmed through infrared spectroscopy analysis (FTIR). Guinea pigs were immunized with free antigen and CTXB antigen or antigen alone by direct administration of antigencontaining CHITOSAN NANOFIBERs into the buccal cavity. Serum immunoglobulin G (IgG) and intestinal immunoglobulin A (IgA) antibody responses were determined Results: The results indicated that antigen in the CHITOSAN NANOFIBERs or NANOCAPSULEs elicited very high IgA and IgG responses. No detectable IgA and IgG responses were obtained after oral immunization with CTXB. The results of the ANTIBODY TITER were analyzed using the ANOVA and LSD tests.Conclusion: CTXB inside the NANOFIBER increased antibody production when administered orally. This system might be used for delivery of other antigens.

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APA: Copy

MASTI, YOUSEF, SAADATI, MOJTABA, NAZARIAN, SHAHRAM, FASIHI RAMANDI, MAHDI, ASADI, MORTEZA, & AREFPOOR, MOHAMMADALI. (2015). PRODUCTION OF ANTIBODY AGAINST CTXB RECOMBINANT PROTEIN BASED ON NANOFIBERS AND NANOCAPSULES. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 17(4), 53-61. SID. https://sid.ir/paper/81280/en

Vancouver: Copy

MASTI YOUSEF, SAADATI MOJTABA, NAZARIAN SHAHRAM, FASIHI RAMANDI MAHDI, ASADI MORTEZA, AREFPOOR MOHAMMADALI. PRODUCTION OF ANTIBODY AGAINST CTXB RECOMBINANT PROTEIN BASED ON NANOFIBERS AND NANOCAPSULES. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2015;17(4):53-61. Available from: https://sid.ir/paper/81280/en

IEEE: Copy

YOUSEF MASTI, MOJTABA SAADATI, SHAHRAM NAZARIAN, MAHDI FASIHI RAMANDI, MORTEZA ASADI, and MOHAMMADALI AREFPOOR, “PRODUCTION OF ANTIBODY AGAINST CTXB RECOMBINANT PROTEIN BASED ON NANOFIBERS AND NANOCAPSULES,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 17, no. 4, pp. 53–61, 2015, [Online]. Available: https://sid.ir/paper/81280/en

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