DESIGN A CHIMERIC PROMOTER CONTAINING SURVIVIN-LIKE CORE PROMOTER IN ORDER TO TRANSCRIPTIONAL TARGETING OF BREAST CANCER

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FAROKHIMANESH S.; RAHBARIZADEH F.; KAMALI ABAS; MOGHADAMPOUR GH.R.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2009 | Volume:12 | Issue:2 Page(s): 73-82

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,517

Download:

Cites:

Information Journal Paper

Title

DESIGN A CHIMERIC PROMOTER CONTAINING SURVIVIN-LIKE CORE PROMOTER IN ORDER TO TRANSCRIPTIONAL TARGETING OF BREAST CANCER

Author(s)

FAROKHIMANESH S. | RAHBARIZADEH F. | KAMALI ABAS | MOGHADAMPOUR GH.R. | Issue Writer Certificate

Keywords

TRANSCRIPTIONAL TARGETINGQ3

SURVIVIN PROMOTERQ3

PROAPOPTOTIC GENEQ3

Abstract

Objective: The greatest challenge in cancer gene therapy is to achieve the high specificity and efficiency in targeting of cancer cells. Because the goal of cancer gene therapy is to eradicate cancer cells, many therapeutic genes could be detrimental if unintentionally expressed in normal cells. Using promoter of the genes which are expressed specifically in cancer cells or have much more expression in cancer cells than normal cells, is very noticeable tool in cancer gene therapy (CGT). In this study we were searching for cancer specific promoter which could highly express therapeutic gene. Materials and methods: In order to apply a cancer specific promoter for creating a CGT construct, a promoter which has 34% similarity to Survivin core promoter was amplified from human genome by using Nested-PCR. Survivin is a member of anti-apoptotic gene and its over-expression was observed in up to 70% of breast cancers. This gene fragment contains two transcriptional binding sites which were similar to SURVIVIN PROMOTER according to the evaluation of Promoter Scan, EPD, Transfac, Compel and TRRD program. These binding sites were recognized by STAT1 and E2F transcription factors. This promoter was cloned into pCDNA3.1/Hygro+ plasmid in along with hypoxia and estrogen modules and pro-apoptotic gene tBid.Results: Semi-quantitative RT-PCR results of transfected cancer cells showed that this gene fragment (Survivin like promoter) have relatively same potential as CMV promoter to direct tBid gene expression.Conclusion: Utilization of chimeric promoter containing Survivin like promoter could be a promising tool in killing cancer cells naturally by inducing apoptosis. This construct is highly effective in TRANSCRIPTIONAL TARGETING of tBid in comparison to control construct.

Cites

No record.

References

No record.

Cite

APA: Copy

FAROKHIMANESH, S., RAHBARIZADEH, F., KAMALI, ABAS, & MOGHADAMPOUR, GH.R.. (2009). DESIGN A CHIMERIC PROMOTER CONTAINING SURVIVIN-LIKE CORE PROMOTER IN ORDER TO TRANSCRIPTIONAL TARGETING OF BREAST CANCER. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 12(2), 73-82. SID. https://sid.ir/paper/81306/en

Vancouver: Copy

FAROKHIMANESH S., RAHBARIZADEH F., KAMALI ABAS, MOGHADAMPOUR GH.R.. DESIGN A CHIMERIC PROMOTER CONTAINING SURVIVIN-LIKE CORE PROMOTER IN ORDER TO TRANSCRIPTIONAL TARGETING OF BREAST CANCER. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2009;12(2):73-82. Available from: https://sid.ir/paper/81306/en

IEEE: Copy

S. FAROKHIMANESH, F. RAHBARIZADEH, ABAS KAMALI, and GH.R. MOGHADAMPOUR, “DESIGN A CHIMERIC PROMOTER CONTAINING SURVIVIN-LIKE CORE PROMOTER IN ORDER TO TRANSCRIPTIONAL TARGETING OF BREAST CANCER,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 12, no. 2, pp. 73–82, 2009, [Online]. Available: https://sid.ir/paper/81306/en

Related Journal Papers