PRODUCTION OF CHIMERIC TIR-INTIMIN PROTEIN FROM E. COLI O157: H7 IN THE TOBACCO (NICOTIANA TOBBACUM) PLANT AND ITS IMMUNOLOGICAL EVALUATION IN AN ANIMAL MODEL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SABERI FARIDEH; SALMANIAN ALI HATEF; AMANI JAFAR; JAFARI MAHYAT

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2012 | Volume:15 | Issue:3 Page(s): 24-36

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

844

Download:

Cites:

Information Journal Paper

Title

PRODUCTION OF CHIMERIC TIR-INTIMIN PROTEIN FROM E. COLI O157: H7 IN THE TOBACCO (NICOTIANA TOBBACUM) PLANT AND ITS IMMUNOLOGICAL EVALUATION IN AN ANIMAL MODEL

Author(s)

SABERI FARIDEH | SALMANIAN ALI HATEF | AMANI JAFAR | JAFARI MAHYAT | Issue Writer Certificate

Keywords

ESCHERICHIA COLI O157: H7Q2

EDIBLE VACCINEQ2

INTIMINQ2

TIRQ2

Abstract

Objective: Escherichia coli (E.coli) O157: H7 is one of the most important pathogenic causes of hemorrhagic colitis in humans. Cattle are the main reservoirs of this bacteria and vaccination is a key mechanism for its control. The INTIMIN, translocated INTIMIN receptor (tir), and EspA proteins are virulence factors expressed by the LEE locus of enterohemorrhagicE. coli. EspA protein is a member of the type III secretion system (TTSS) needle complexes that delivers the TIR protein into the host cell. Surface arrayed INTIMIN docks the bacterium to the translocated INTIMIN receptor (Tir). This intimate linkage is the starting point for attachment and effacing lesions. We hypothesize that the chimeric recombinant forms of two of these three effectors, as edible-based immunogens, would reduce colonization of E. coli O157: H7 in the mice model.Methods: We constructed a synthetic gene (it) composed of eae (i) and TIR (t) attached together by a peptide linker. The synthetic gene (it) was codon optimized based on the tobacco (Nicotiana tobbacum) plant and cloned into plant expression vectors adjacent to CaMV35S promoters for expression in transgenic tobacco plants. The antigen produced in this plant was orally fed to mice.Results: Immunization of the mice model by the transgenic plant that contained the divalent immunogen showed the presence of IgG antibodies against E. coli O157: H7.Conclusion: This method could be an effective tool for protecting against E. coli O157: H7 hemorrhagic colitis.

Cites

No record.

References

No record.

Cite

APA: Copy

SABERI, FARIDEH, SALMANIAN, ALI HATEF, AMANI, JAFAR, & JAFARI, MAHYAT. (2012). PRODUCTION OF CHIMERIC TIR-INTIMIN PROTEIN FROM E. COLI O157: H7 IN THE TOBACCO (NICOTIANA TOBBACUM) PLANT AND ITS IMMUNOLOGICAL EVALUATION IN AN ANIMAL MODEL. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 15(3), 24-36. SID. https://sid.ir/paper/81320/en

Vancouver: Copy

SABERI FARIDEH, SALMANIAN ALI HATEF, AMANI JAFAR, JAFARI MAHYAT. PRODUCTION OF CHIMERIC TIR-INTIMIN PROTEIN FROM E. COLI O157: H7 IN THE TOBACCO (NICOTIANA TOBBACUM) PLANT AND ITS IMMUNOLOGICAL EVALUATION IN AN ANIMAL MODEL. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2012;15(3):24-36. Available from: https://sid.ir/paper/81320/en

IEEE: Copy

FARIDEH SABERI, ALI HATEF SALMANIAN, JAFAR AMANI, and MAHYAT JAFARI, “PRODUCTION OF CHIMERIC TIR-INTIMIN PROTEIN FROM E. COLI O157: H7 IN THE TOBACCO (NICOTIANA TOBBACUM) PLANT AND ITS IMMUNOLOGICAL EVALUATION IN AN ANIMAL MODEL,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 15, no. 3, pp. 24–36, 2012, [Online]. Available: https://sid.ir/paper/81320/en

Related Journal Papers