EFFECT OF BETA-MERCAPTOETHANOL ON IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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IMANI H.; HASANI F.; HAERI ROUHANI SEYED ALI; NASRESFAHANI MOHAMMAD HOSSEIN; DALMAN A.; REZAZADEH M.; KAZEMI ASHTIANI S.; SHAHVERDI A.; BAHARVAND H.

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Journal: Anatomical Sciences Journal Year:2004 | Volume:2 | Issue:1 Page(s): 37-45

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Title

EFFECT OF BETA-MERCAPTOETHANOL ON IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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Keywords

IMMATURE OOCYTEQ3

BETA -MERCAPTOETHANOLQ4

MOUSEQ3

Abstract

Purpose: This Study evaluated the resumption of meiosis, in vitro maturtion and department of immature MOUSE oocytes with b- mercaptothanol.Materials and methods: IMMATURE OOCYTEs were recovered from NMRI MOUSE strain (4-6 weeks) in different 4 experiments groups: the control group, the oocytes were matured in MEM-a+ medium containing + FCS 55%; group 1: the oocytes were matured in MEM- a medium containing + FCS 5% and b-mercaptoethanol; group 2: the oocytes were matured in MEM-a+ FCS 5%, rFSH 100mIU and group 3: the oocytes were mature in MEM- a medium containing, FCS 5%, hCG 7.5IU, rFSH100mIU, b-mer-captoethanol 100 mm. Fertilization and embryo development were done in T6 medium.Results: There were significant differences on in vitro maturation between control group than the group 1 and group 3 (p=0.01, p=0.007) and resumption of miosis between control group than the group 1 and group 3 (p=0.009, p=0.001).The rates of the resumpsion of meiosis in experimental groups were 74.20 (control), 82.71 (group 1), 84.49 (group 2) and 85.49% (group 3), In vitro maturation also increased significantly with (3-mercapthoethanol (group 1=69.49%, group 3=70.22%).The embryo formation on the fourth day in group 1 than the control group, group 2, group 3 (p=0.001, p=0.000,p=0.000) increased more significantly than that in the other groups.Conclusion: The results of this study showed that b-mer-captoethanol (100 mm)has an effect on in vitro maturation and resumption of meiosis of MOUSE oocytes and improves embryo development.

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APA: Copy

IMANI, H., HASANI, F., HAERI ROUHANI, SEYED ALI, NASRESFAHANI, MOHAMMAD HOSSEIN, DALMAN, A., REZAZADEH, M., KAZEMI ASHTIANI, S., SHAHVERDI, A., & BAHARVAND, H.. (2004). EFFECT OF BETA-MERCAPTOETHANOL ON IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 2(1), 37-45. SID. https://sid.ir/paper/81370/en

Vancouver: Copy

IMANI H., HASANI F., HAERI ROUHANI SEYED ALI, NASRESFAHANI MOHAMMAD HOSSEIN, DALMAN A., REZAZADEH M., KAZEMI ASHTIANI S., SHAHVERDI A., BAHARVAND H.. EFFECT OF BETA-MERCAPTOETHANOL ON IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2004;2(1):37-45. Available from: https://sid.ir/paper/81370/en

IEEE: Copy

H. IMANI, F. HASANI, SEYED ALI HAERI ROUHANI, MOHAMMAD HOSSEIN NASRESFAHANI, A. DALMAN, M. REZAZADEH, S. KAZEMI ASHTIANI, A. SHAHVERDI, and H. BAHARVAND, “EFFECT OF BETA-MERCAPTOETHANOL ON IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 2, no. 1, pp. 37–45, 2004, [Online]. Available: https://sid.ir/paper/81370/en

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