STUDY OF DIFFERENTIATION POTENTIAL OF THE DEDIFFERENTIATED CHONDROCYTES FROM RAT ARTICULAR CARTILAGE INTO SKELETAL CELL LINEAGES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAGHBAN ESLAMINEZHAD M.R.; TAGHIYAR LEYLA; FALAHI FAHIMEH; AZHDARI TAFTI Z.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Anatomical Sciences Journal Year:2008 | Volume:6 | Issue:24 Page(s): 435-445

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

814

Download:

Cites:

Information Journal Paper

Title

STUDY OF DIFFERENTIATION POTENTIAL OF THE DEDIFFERENTIATED CHONDROCYTES FROM RAT ARTICULAR CARTILAGE INTO SKELETAL CELL LINEAGES

Author(s)

BAGHBAN ESLAMINEZHAD M.R. | TAGHIYAR LEYLA | FALAHI FAHIMEH | AZHDARI TAFTI Z. | Issue Writer Certificate

Keywords

CHONDROCYTESQ3

ARTICULAR CARTILAGEQ2

DEDIFFERENTIATIONQ2

DIFFERENTIATION INTO SKELETAL CELL LINEAGESQ3

Abstract

Purpose: DEDIFFERENTIATION of the chondrocyte from rat ARTICULAR CARTILAGE with multiple subcultures and study of the redifferentiation potential of the cells into bone, cartilage and fat cell lineages.Materials and Methods: In this experimental study, CHONDROCYTES from rat ARTICULAR CARTILAGE were isolated and expanded through several successive subcultures during which the expression levels of cartilage-specific genes including aggreacan and type II collagen were measured by using real-time PCR to determine the cell DEDIFFERENTIATION (the time in which cartilage genes ceased their expression). Furthermore, during the culture period, the chondrocyte was examined morphologically by scanning electron microscopy (SEM). At the end, the dedifferentiated cells were subjected to osteogenic, adipogenic and chondrogenic culture condition to investigate whether or not they are able to redifferentaite into specialized progenies. Differentiation state was examined by specific staining and RT-PCR analysis.Results: Based on the findings by real time PCR, the expression levels of the both studied genes were high at passage 2 and dramatically decreased at passage 4. Aggreacan expression ceased at passage 10 and collagen II stopped expressing at passage 6. SEM images indicated the flattened morphology of the cells at early passages and the fibroblastic appearance at late passages. Differentiation examination revealed that the dedifferentiated cells were readily differentiated into bone, adipose and cartilage cell lineages. Conclusion: Considering all aspects together, this concluded that articular chondrocyte gradually lost their differentiated state during the long-term culture and changed into multipotent cells capable of differentiating into skeletal cell lineages.

Cites

No record.

References

No record.

Cite

APA: Copy

BAGHBAN ESLAMINEZHAD, M.R., TAGHIYAR, LEYLA, FALAHI, FAHIMEH, & AZHDARI TAFTI, Z.. (2008). STUDY OF DIFFERENTIATION POTENTIAL OF THE DEDIFFERENTIATED CHONDROCYTES FROM RAT ARTICULAR CARTILAGE INTO SKELETAL CELL LINEAGES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 6(24), 435-445. SID. https://sid.ir/paper/81402/en

Vancouver: Copy

BAGHBAN ESLAMINEZHAD M.R., TAGHIYAR LEYLA, FALAHI FAHIMEH, AZHDARI TAFTI Z.. STUDY OF DIFFERENTIATION POTENTIAL OF THE DEDIFFERENTIATED CHONDROCYTES FROM RAT ARTICULAR CARTILAGE INTO SKELETAL CELL LINEAGES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2008;6(24):435-445. Available from: https://sid.ir/paper/81402/en

IEEE: Copy

M.R. BAGHBAN ESLAMINEZHAD, LEYLA TAGHIYAR, FAHIMEH FALAHI, and Z. AZHDARI TAFTI, “STUDY OF DIFFERENTIATION POTENTIAL OF THE DEDIFFERENTIATED CHONDROCYTES FROM RAT ARTICULAR CARTILAGE INTO SKELETAL CELL LINEAGES,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 6, no. 24, pp. 435–445, 2008, [Online]. Available: https://sid.ir/paper/81402/en

Related Journal Papers