EFFICIENT METHOD FOR COMBINED ELECTRICAL-CHEMICAL PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES

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NASRESFAHANI MOHAMMAD HOSSEIN; HAJIAN MAHDI; HOSSEINI SEYED MORTEZA; MOULAVI F.; FOROUZANFAR MOHSEN; ABEDI PARVANEH; MEMAR EMAMIEH M.; PILEHVARIAN A.A.; SHAHVERDI A.A.H.

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Journal: Anatomical Sciences Journal Year:2007 | Volume:4 | Issue:17 Page(s): 345-358

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Title

EFFICIENT METHOD FOR COMBINED ELECTRICAL-CHEMICAL PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES

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Keywords

BOVINEQ3

OOCYTEQ3

PARTHENOGENETIC ACTIVATIONQ2

EMBRYO DEVEQ3

Abstract

Purpose: PARTHENOGENETIC ACTIVATION is among the crucial steps determining successful development of mammalian cloned embryos. This study, therefore, was conducted to evaluate the efficiency of a novel combined electrical-chemical artificial activation method for BOVINE cloning.Materials and Methods: In vitro matured BOVINE OOCYTEs than were initially exposed to electrical pulse, used for cell fusion during cloning, and then treated with temporal sequential combinations of 3 chemical activators [calcium ionophore (CI), strontium (SR) and ethanol (ET)], followed by exposure to a protein kinase inhibitor or used for in vitro fertilization as control group. Treated and naturally fertilized OOCYTEs were further cultured for up to 8 days. EMBRYO DEVElopment was scored daily and blastocyst cell counting carried out using differential staining at day 8 of culture.Results: Among 15 temporal sequential combinations of three chemical activators, the best cleavage rates were associated with double (SR CI, 84.4%), triple (CI SR ET, 79.4%) and single (CI, 73.7%) compounds, respectively, which were not significantly different with each other and with in vitro fertilized (IVF) (85.5%). The highest blastocyst rates were gained with ET SR (25%), SR CI ET (21.7%) and CI (20.3%) accordingly which were not significantly different with each other but significantly lower than IVF (46%).Embryo cell counting further confirmed reasonably better quality of blastocysts produced using double, triple and single compounds.Conclusion: Although most of the sequential artificial activation compounds induced high cleavage rate close to IVF, but this did not assure comparable further EMBRYO DEVElopment to the blastocyst stage.Nevertheless, the results suggest exposure of in vitro matured BOVINE OOCYTEs to electrical pulse, followed by exposure to CI 6- dimethylaminopurine (6-DMAP) or ET SR 6-DMAP could be regarded as the optimal artificial activation.

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APA: Copy

NASRESFAHANI, MOHAMMAD HOSSEIN, HAJIAN, MAHDI, HOSSEINI, SEYED MORTEZA, MOULAVI, F., FOROUZANFAR, MOHSEN, ABEDI, PARVANEH, MEMAR EMAMIEH, M., PILEHVARIAN, A.A., & SHAHVERDI, A.A.H.. (2007). EFFICIENT METHOD FOR COMBINED ELECTRICAL-CHEMICAL PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 4(17), 345-358. SID. https://sid.ir/paper/81535/en

Vancouver: Copy

NASRESFAHANI MOHAMMAD HOSSEIN, HAJIAN MAHDI, HOSSEINI SEYED MORTEZA, MOULAVI F., FOROUZANFAR MOHSEN, ABEDI PARVANEH, MEMAR EMAMIEH M., PILEHVARIAN A.A., SHAHVERDI A.A.H.. EFFICIENT METHOD FOR COMBINED ELECTRICAL-CHEMICAL PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2007;4(17):345-358. Available from: https://sid.ir/paper/81535/en

IEEE: Copy

MOHAMMAD HOSSEIN NASRESFAHANI, MAHDI HAJIAN, SEYED MORTEZA HOSSEINI, F. MOULAVI, MOHSEN FOROUZANFAR, PARVANEH ABEDI, M. MEMAR EMAMIEH, A.A. PILEHVARIAN, and A.A.H. SHAHVERDI, “EFFICIENT METHOD FOR COMBINED ELECTRICAL-CHEMICAL PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 4, no. 17, pp. 345–358, 2007, [Online]. Available: https://sid.ir/paper/81535/en

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