A MODIFIED ELECTROCHEMICAL ELECTRODE FOR DEVELOPMENT OF NANOBIOSENSOR FOR THE DETECTION OF RFBE GENE OF ESCHERICHIA COLI O157:H7

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MINAEI MOHAMMAD EBRAHIM; SAADATI MOJTABA; NAJAFI MOSTAFA; HONARI HOSSEIN

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Paper Information

Journal: Journal of Sabzevar University of Medical Sciences Year:2015 | Volume:22 | Issue:5 Page(s): 797-804

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Title

A MODIFIED ELECTROCHEMICAL ELECTRODE FOR DEVELOPMENT OF NANOBIOSENSOR FOR THE DETECTION OF RFBE GENE OF ESCHERICHIA COLI O157:H7

Author(s)

MINAEI MOHAMMAD EBRAHIM | SAADATI MOJTABA | NAJAFI MOSTAFA | HONARI HOSSEIN | Issue Writer Certificate

Keywords

DNA HYBRIDIZATIONQ2

ESCHERICHIA COLI O157:H7Q2

NANOBIOSENSORQ2

Abstract

Background & Objectives: NANOBIOSENSORs could be used instead of traditional detection methods of ESCHERICHIA COLI O157:H7. In this paper, a NANOBIOSENSOR for detection of rfbE gene of the ESCHERICHIA COLI O157:H7 has been studied by immobilization and hybridization single strand DNA (ssDNA) sequences on the electrode surface modified with gold nanoparticles.Materials & Methods: Electrochemical impedance spectroscopy technique was used to study the properties of the sensing modified electrode. The working electrode surface was modified by electrochemical method with gold nanoparticles. The single-stranded DNA sequence using self-assembled monolayer was immobilized on the gold electrode for two hours. A mixed monolayer comprising both mercaptohexanol (MCH) and mercaptopropionic acid (MPA) were used as blocking layer. The hybridization DNA/DNA was performed by immersion of the modified gold electrode into ssDNA at a concentration of 1 mM target DNA.Results: The results showed that the electrode modified with gold nanoparticles after immobilizing ssDNA effectively detected rfbE gene of ESCHERICHIA COLI O157:H7 by DNA HYBRIDIZATION. The nanobiosiosensor showed suitable selectivity for the detection of target DNA complementary sequence compared with the mismatched oligonucleotides sequence and the noncomplementary oligonucleotides sequence.Conclusion: According to the results obtained and similar studies, the electrochemical NANOBIOSENSOR based on DNA HYBRIDIZATION has advantages such as low cost, simplicity, and miniaturization and can provide a basis for the development of genomic detection tools.

Cites

THE NANOBIOSENSOR BASED ON DNA HYBRIDIZATION FOR THE DETECTION OF RFBE GENE IN REAL SAMPLES OF ESCHERICHIA COLI O157:H7

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APA: Copy

MINAEI, MOHAMMAD EBRAHIM, SAADATI, MOJTABA, NAJAFI, MOSTAFA, & HONARI, HOSSEIN. (2015). A MODIFIED ELECTROCHEMICAL ELECTRODE FOR DEVELOPMENT OF NANOBIOSENSOR FOR THE DETECTION OF RFBE GENE OF ESCHERICHIA COLI O157:H7. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 22(5), 797-804. SID. https://sid.ir/paper/81847/en

Vancouver: Copy

MINAEI MOHAMMAD EBRAHIM, SAADATI MOJTABA, NAJAFI MOSTAFA, HONARI HOSSEIN. A MODIFIED ELECTROCHEMICAL ELECTRODE FOR DEVELOPMENT OF NANOBIOSENSOR FOR THE DETECTION OF RFBE GENE OF ESCHERICHIA COLI O157:H7. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2015;22(5):797-804. Available from: https://sid.ir/paper/81847/en

IEEE: Copy

MOHAMMAD EBRAHIM MINAEI, MOJTABA SAADATI, MOSTAFA NAJAFI, and HOSSEIN HONARI, “A MODIFIED ELECTROCHEMICAL ELECTRODE FOR DEVELOPMENT OF NANOBIOSENSOR FOR THE DETECTION OF RFBE GENE OF ESCHERICHIA COLI O157:H7,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 5, pp. 797–804, 2015, [Online]. Available: https://sid.ir/paper/81847/en

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