MOLECULAR DETECTION OF THE PATHOGENIC HLYA GENE AND ANTIBIOTIC SUSCEPTIBILITY IN 100 SPECIMENS FROM PATIENTS WITH CHOLERA

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SEDAGHAT M.; DORANI S.S.; POURSHAFIEI M.R.; SEYFI MAHNAZ; RANJBAR REZA

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Journal: JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH Year:2006 | Volume:4 | Issue:3 Page(s): 53-60

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Title

MOLECULAR DETECTION OF THE PATHOGENIC HLYA GENE AND ANTIBIOTIC SUSCEPTIBILITY IN 100 SPECIMENS FROM PATIENTS WITH CHOLERA

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Keywords

VIBRIO CHOLERA EL TORQ4

POLYMERASE CHAIN REACTIONQ2

HEMOLYSISQ4

Abstract

Background and Aim: Cholera is one of the most important endemic and epidemic diseases in our country. Therefore, keeping track of the changes in antibiotic resistance patterns and continuous genotyping and serotyping of the isolates is essential.Materials and Methods: One hundred stool samples were collected from the patients with cholera in different cities, including Tehran, Kashan, Kermanshah and Ahwaz. The specimens were subjected to a series of diagnostic tests. Strains were identified by serotyping and their antibiotic resistance profiles were defined through susceptibility tests. We also used a POLYMERASE CHAIN REACTION (PCR) assay to identify the hlyA gene.Results: Group-specific antisera were used for identification of Ogawa, Inaba, Hikojima and NAG (Non-agglutinable) strains, which constituted 74%, 3%, 0% and 23% of the isolated strains respectively. Antibiotic susceptibility tests showed that all the strains were sensitive to ciprofloxacin, gentamicin and doxycyclin, but there was widespread resistance to Co-trimoxazol (74%), erythromycin (64%) and tetracycline (53%). Vibrio cholerae E1 for isolates were 100% positive for hlyA gene amplification, but hemolytic activity was seen in 95% of the cases on blood agar.Conclusion: Compared to previous studies, there appears to be a higher degree of resistance to tetracycline, hence there may be a need to switch to other agents for the treatment of cholera. The predominant serotype and the antibiotic resistance pattern in Kashan were different from those seen in other cities.

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APA: Copy

SEDAGHAT, M., DORANI, S.S., POURSHAFIEI, M.R., SEYFI, MAHNAZ, & RANJBAR, REZA. (2006). MOLECULAR DETECTION OF THE PATHOGENIC HLYA GENE AND ANTIBIOTIC SUSCEPTIBILITY IN 100 SPECIMENS FROM PATIENTS WITH CHOLERA. JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH, 4(3), 53-60. SID. https://sid.ir/paper/85119/en

Vancouver: Copy

SEDAGHAT M., DORANI S.S., POURSHAFIEI M.R., SEYFI MAHNAZ, RANJBAR REZA. MOLECULAR DETECTION OF THE PATHOGENIC HLYA GENE AND ANTIBIOTIC SUSCEPTIBILITY IN 100 SPECIMENS FROM PATIENTS WITH CHOLERA. JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH[Internet]. 2006;4(3):53-60. Available from: https://sid.ir/paper/85119/en

IEEE: Copy

M. SEDAGHAT, S.S. DORANI, M.R. POURSHAFIEI, MAHNAZ SEYFI, and REZA RANJBAR, “MOLECULAR DETECTION OF THE PATHOGENIC HLYA GENE AND ANTIBIOTIC SUSCEPTIBILITY IN 100 SPECIMENS FROM PATIENTS WITH CHOLERA,” JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH, vol. 4, no. 3, pp. 53–60, 2006, [Online]. Available: https://sid.ir/paper/85119/en

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