DIFFERENTIAL DIAGNOSIS OF ENTAMOEBA HISTOLYTICA FROM ENTAMOEBA DISPAR AND A STUDY ON THE INTESTINAL PARASITES IN RURAL AREAS OF AHWAZ AND HAMIDIEH

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REZAEIAN MOSTAFA; HOUSHYAR HOSSEIN

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Journal: JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH Year:2007 | Volume:4 | Issue:4 Page(s): 33-38

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Title

DIFFERENTIAL DIAGNOSIS OF ENTAMOEBA HISTOLYTICA FROM ENTAMOEBA DISPAR AND A STUDY ON THE INTESTINAL PARASITES IN RURAL AREAS OF AHWAZ AND HAMIDIEH

Author(s)

REZAEIAN MOSTAFA | HOUSHYAR HOSSEIN | Issue Writer Certificate

Keywords

ENTAMOEBA HISTOLYTICAQ4

ENTAMOEBA DISPARQ4

INTESTINAL PARASITEQ4

AHWAZQ2

Abstract

Background and Aim: Differential diagnosis of ENTAMOEBA HISTOLYTICA and ENTAMOEBA DISPAR is important in clinical and epidemiological studies. The two organisms are morphologically identical but they differ in their genetics, biochemistry, and pathogenicity. The present study was carried out with the aim of distinguishing the two species and determining the prevalence of each organism in the rural areas of AHWAZ and Hamidieh.Material and Methods: A total of 782 stool specimens were randomly collected and examined by formalin-ether concentration and direct methods. Twenty-one isolates of E. histolytical E. dispar were successfully cultured on Robinson's medium. DNA was extracted by the phenol/chloroformmethod and identified by PCR-RFLP after digestion with HinfI.Results: Over 75% of the individuals were infected with at least one of the INTESTINAL PARASITEs. Entamoeba coli infection rates were very high (51.9%) among the population, while only 0.76% of individuals were positive for Dientamoeba fragile. Sixty-five individual (8/3%) were infected with E. histolytica /E. dispar.The PCR-RFLP showed that 19 samples (90.48%) were positive for E. dispar; one sample (4.76%) was positive for E. histolytica and another sample (4.76%) showed mixed infection.Conclusion these findings show that the nonpathogenic E. dispar is predominant in AHWAZ and Hamidieh rural area.

Cites

IDENTIFICATION OF ENTAMOEBA HISTOLYTICA BY MOLECULAR METHOD IN SURFACE WATER OF RASHT CITY, IRAN

References

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APA: Copy

REZAEIAN, MOSTAFA, & HOUSHYAR, HOSSEIN. (2007). DIFFERENTIAL DIAGNOSIS OF ENTAMOEBA HISTOLYTICA FROM ENTAMOEBA DISPAR AND A STUDY ON THE INTESTINAL PARASITES IN RURAL AREAS OF AHWAZ AND HAMIDIEH. JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH, 4(4), 33-38. SID. https://sid.ir/paper/85203/en

Vancouver: Copy

REZAEIAN MOSTAFA, HOUSHYAR HOSSEIN. DIFFERENTIAL DIAGNOSIS OF ENTAMOEBA HISTOLYTICA FROM ENTAMOEBA DISPAR AND A STUDY ON THE INTESTINAL PARASITES IN RURAL AREAS OF AHWAZ AND HAMIDIEH. JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH[Internet]. 2007;4(4):33-38. Available from: https://sid.ir/paper/85203/en

IEEE: Copy

MOSTAFA REZAEIAN, and HOSSEIN HOUSHYAR, “DIFFERENTIAL DIAGNOSIS OF ENTAMOEBA HISTOLYTICA FROM ENTAMOEBA DISPAR AND A STUDY ON THE INTESTINAL PARASITES IN RURAL AREAS OF AHWAZ AND HAMIDIEH,” JOURNAL OF SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF PUBLIC HEALTH RESEARCH, vol. 4, no. 4, pp. 33–38, 2007, [Online]. Available: https://sid.ir/paper/85203/en

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