EFFECTS OF MICRO-FILTER IN DETECTION OF COLIFORM IN TAP WATER BY PCR

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GHANADZADEH M.J.; ABTAHI HAMID; SALMANIAN ALI HATEF; GHAZNAVIRAD E.A.; KARIMI MASOUMEH

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Journal: Journal of Ilam University of Medical Sciences Year:2009 | Volume:17 | Issue:3 Page(s): 10-15

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Title

EFFECTS OF MICRO-FILTER IN DETECTION OF COLIFORM IN TAP WATER BY PCR

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Keywords

COLIFORMQ3

PCRQ3

MICROFILTERQ3

Abstract

Introduction: For detection of E.coli in top water by PCR, the samples must be concentrated by filter. In this study, we investigated two kinds of micro filters in PCR results. Materials & Methods: After preparing dilution of E.coli (8.100, 4.100, 2.100, 1.100, 1.200, 1.400, 1.800 and 1.1600) in D.W, the bacteria were separated by FHLP and HAWP filters and 16s rRNA was propagated by the above- mentioned primers.Findings: The rate of bacteria in dilutions was confirmed by culture. The PCR data showed that FHLP, better than HAWP filter, would be able to separate the bacteria in such dilutions after filtration. Discussion & Conclusion: The results of this study showed that hydrophobic filter (FHLP) has a higher ability than that of hydrophilic filter (HAWP) in separating bacteria. So, successful PCR amplifications were achieved by cells concentrated with hydrophobic filters for detection of all the COLIFORM bacteria, while false negative results decreased considerably.

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APA: Copy

GHANADZADEH, M.J., ABTAHI, HAMID, SALMANIAN, ALI HATEF, GHAZNAVIRAD, E.A., & KARIMI, MASOUMEH. (2009). EFFECTS OF MICRO-FILTER IN DETECTION OF COLIFORM IN TAP WATER BY PCR. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 17(3), 10-15. SID. https://sid.ir/paper/89942/en

Vancouver: Copy

GHANADZADEH M.J., ABTAHI HAMID, SALMANIAN ALI HATEF, GHAZNAVIRAD E.A., KARIMI MASOUMEH. EFFECTS OF MICRO-FILTER IN DETECTION OF COLIFORM IN TAP WATER BY PCR. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2009;17(3):10-15. Available from: https://sid.ir/paper/89942/en

IEEE: Copy

M.J. GHANADZADEH, HAMID ABTAHI, ALI HATEF SALMANIAN, E.A. GHAZNAVIRAD, and MASOUMEH KARIMI, “EFFECTS OF MICRO-FILTER IN DETECTION OF COLIFORM IN TAP WATER BY PCR,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 17, no. 3, pp. 10–15, 2009, [Online]. Available: https://sid.ir/paper/89942/en

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