Metallo-beta-Lactamase Producing Pseudomonas aeruginosa

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Naserian moghadam R.; Roodbari F.; NADERI NASAB M.; MANSOURI D.; MIRBAGHERI Z.; GHOLOOBI A.; Chichaklu A.; MESHKAT Z.

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Title

Metallo-beta-Lactamase Producing Pseudomonas aeruginosa

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Keywords

Metallo-beta-lactamaseQ1

Pseudomonas aeruginosaQ2

Antibiotic resistanceQ1

VIM geneQ1

Abstract

Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of Metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes. Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method. Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24. 5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12. 5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples. Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest Antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance.

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APA: Copy

Naserian moghadam, R., Roodbari, F., NADERI NASAB, M., MANSOURI, D., MIRBAGHERI, Z., GHOLOOBI, A., Chichaklu, A., & MESHKAT, Z.. (2018). Metallo-beta-Lactamase Producing Pseudomonas aeruginosa. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 26(4 ), 43-52. SID. https://sid.ir/paper/90203/en

Vancouver: Copy

Naserian moghadam R., Roodbari F., NADERI NASAB M., MANSOURI D., MIRBAGHERI Z., GHOLOOBI A., Chichaklu A., MESHKAT Z.. Metallo-beta-Lactamase Producing Pseudomonas aeruginosa. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;26(4 ):43-52. Available from: https://sid.ir/paper/90203/en

IEEE: Copy

R. Naserian moghadam, F. Roodbari, M. NADERI NASAB, D. MANSOURI, Z. MIRBAGHERI, A. GHOLOOBI, A. Chichaklu, and Z. MESHKAT, “Metallo-beta-Lactamase Producing Pseudomonas aeruginosa,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 4 , pp. 43–52, 2018, [Online]. Available: https://sid.ir/paper/90203/en

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