BIODIESEL PRODUCTION FROM OLIVE OIL BY IMMOBILIZED LIPASE

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GHARAGAZLOO SOROOR; AGHAEI HAMIDREZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Seminar Paper

Paper Information

Seminar: IRANIAN CHEMISTRY CONGRESS
Year:2014 | Issue:17

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Title

BIODIESEL PRODUCTION FROM OLIVE OIL BY IMMOBILIZED LIPASE

Author(s)

GHARAGAZLOO SOROOR | AGHAEI HAMIDREZA | Issue Writer Certificate

Keywords

LIPASE

TRANS ESTERIFICATION

BIODIESEL

OLIVE OIL

Abstract

BACKGROUND: NOWADAYS, VEGETABLE USED OILS ARE A POTENTIAL RENEWABLE SOURCE FOR THE PRODUCTION OF AN ALTERNATIVE FOR PETROLEUM BASED DIESEL FUEL DUE TO THE DIMINISHING PETROLEUM RESERVES AND THE ENVIRONMENTAL CONSEQUENCES OF EXHAUST GASES FROM DIESEL ENGINES. THE BIODIESEL PRODUCTION FROM VEGETABLE OILS INVOLVES THE TRANS ESTERIFICATION OF VEGETABLE OILS USING A SHORT CHAIN ALCOHOL IN THE PRESENCE OF A SUITABLE CATALYST OBTAINING A FUEL QUITE SIMILAR TO CONVENTIONAL DIESEL FUEL IN ITS MAIN CHARACTERISTICS [1]. ENZYMATIC BIODIESEL PRODUCTION FROM RAW VEGETABLE OILS HAS BEEN EXTENSIVELY STUDIED FOR MANY AUTHORS IN THE LAST YEARS [2-4]. IN THIS STUDY, OLIVE OIL WAS USED AS FOR ENZYMATIC BIODIESEL PRODUCTION. THE PURPOSE OF THIS WORK IS TO ANALYZE THE INFLUENCE OF OPERATING PARAMETERS, SUCH AS METHANOL TO OIL MOLAR RATIO, DOSAGE OF ENZYME, REACTION TIME AND ENZYME REUSE ON TRANS ESTERIFICATION PROCESS.METHODS: THE ACTIVATION OF HYDROXYL GROUPS OF CLOISITE WAS ACHIEVED BY REACTION WITH P-TOLUENESULFONYL CHLORIDE. FOR PREPARATION OF AMINATED CLOISITE, TOSYLATED CLOISITE WAS DISPERSED IN A FLASK CONTAINING HEXAMETHYLENE DIAMINE. THE ACTIVATION OF AMINO GROUPS OF THE ACTIVATED CLOISITE WAS ACHIEVED BY REACTION WITH EPICHLOROHYDRIN. FOR IMMOBILIZATION OF LIPASE, ACTIVATED CLOISITE WERE EQUILIBRATED IN PHOSPHATE BUFFER AND TRANSFERRED TO THE SAME FRESH MEDIUM CONTAINING LIPASE. THE ENZYMATIC TRANS ESTERIFICATION REACTIONS WERE CARRIED OUT IN A TEST TUBE CONTAINING 0.1 ML OF OLIVE OIL, 500 MG OF IMMOBILIZED LIPASE AND METHANOL. THE MOLAR AMOUNT OF THE OIL WAS CALCULATED FROM THE ACID VALUE. THE REACTION WAS CARRIED OUT IN AN INCUBATOR AT 50OC DURING 4 H WITH CONSTANT STIRRING.RESULTS: AT THIS STUDY, EFFECTS OF TEMPERATURE, REACTION TIME, MOLAR RATIO (METHANOL TO OIL) AND TYPE OF SOLVENT ARE INVESTIGATED. REMARKABLY, THE OPTIMUM REACTION CONDITIONS ARE METHANOL TO OIL MOLAR RATIO OF 10:1, REACTION PERIOD OF 4 H AT 50OC IN N-HEXANE.CONCLUSION: IMMOBILIZED LIPASE CATALYZED BIODIESEL PRODUCTION ALLOWS THE USE OF MILD AND MORE ENVIRONMENTALLY FRIENDLY REACTION CONDITIONS. THE RESULTS INDICATED THAT THROUGH THE OPTIMIZATION OF REACTION CONDITIONS, BIODIESEL WAS OBTAINED IN ALMOST 60% AND THERE WAS NO OBVIOUS LOSS IN THE YIELD OF BIODIESEL AFTER BEING CONSECUTIVELY USED FOR SIX CYCLES IN THE TRANS ESTERIFICATION REACTIONS USING IMMOBILIZED LIPASE.

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APA: Copy

GHARAGAZLOO, SOROOR, & AGHAEI, HAMIDREZA. (2014). BIODIESEL PRODUCTION FROM OLIVE OIL BY IMMOBILIZED LIPASE. IRANIAN CHEMISTRY CONGRESS. SID. https://sid.ir/paper/909217/en

Vancouver: Copy

GHARAGAZLOO SOROOR, AGHAEI HAMIDREZA. BIODIESEL PRODUCTION FROM OLIVE OIL BY IMMOBILIZED LIPASE. 2014. Available from: https://sid.ir/paper/909217/en

IEEE: Copy

SOROOR GHARAGAZLOO, and HAMIDREZA AGHAEI, “BIODIESEL PRODUCTION FROM OLIVE OIL BY IMMOBILIZED LIPASE,” presented at the IRANIAN CHEMISTRY CONGRESS. 2014, [Online]. Available: https://sid.ir/paper/909217/en

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