SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND CYSTEINE BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER BETWEEN GOLD NANOPARTICLES AND FITC

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HORMOZI NEZHAD M.REZA; MOHAMADI J.

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Seminar Paper

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Seminar: IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY
Year:2014 | Issue:20

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Title

SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND CYSTEINE BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER BETWEEN GOLD NANOPARTICLES AND FITC

Author(s)

HORMOZI NEZHAD M.REZA | MOHAMADI J. | Issue Writer Certificate

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Abstract

TURN-ON FLUORESCENCE DETECTION IS A SELECTIVE AND SENSITIVE METHOD FOR MANY ANALYTICAL PURPOSES. FLUORESCENCE QUENCHING IS A COMMONLY OBSERVED CONSEQUENCE WHEN FLUOROPHORES AND GOLD NANOPARTICLES (AU NPS) ARE IN SAME MEDIA. HERE, WE REPORT A “TURN-ON” FLUORESCENT NANOPROBE FOR SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND CYSTEINE USING H-POINT STANDARD ADDITION METHOD (HPSAM). THIS PROBE DEVELOPS BASED ON THE FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET) BETWEEN AU NPS AND FLUORESCEIN ISOTHIOCYANATE (FITC), IN WHICH FITC ACTS AS THE DONOR AND AU NPS AS THE ACCEPTOR. FRET, ELECTRON TRANSFER PROCESSES AND INNER FILTER EFFECT (IFE) CAUSE THE FLUORESCENCE INTENSITY OF FITC STRICTLY QUENCHED AS A RESULT OF NONCOVALENTLY ADSORBED ON AUNPS SURFACE [1, 2]. THIS PROBE OPERATED ON A BASIC PRINCIPLE THAT GLUTATHIONE AND CYSTEINE HAVE STRONGER AFFINITY TO AU NPS, SO DEPOSIT ON THE SURFACES OF THE AU NPS AND INDUCE THE RELEASE OF FITC MOLECULES INTO THE SOLUTION WITH DIFFERENT RATES, AND THUS RESTORED THE FLORESCENCE SIGNAL OF FITC [2, 3]. WE TRY TO OMIT IFE SIGNAL IN EVERY PART OF OUR WORK USING UVVIS SPECTRA AND MATHEMATICAL CALCULATIONS. THE H-POINT STANDARD ADDITION METHOD (HPSAM) WAS APPLIED TO KINETIC DATA FOR SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND CYSTEINE. UNDER THE OPTIMUM CONDITIONS (I.E. PH=8.5, CAUNPS=0.6 NM), THE FLUORESCENCE INTENSITY OF THE RELEASED FITC DISPLAYS A LINEAR RELATIONSHIP IN THE RANGE OF 20 TO 200 NM OF GLUTATHIONE IN THE PRESENCE OF CYSTEINE. THE PROPOSED METHOD WAS SUCCESSFULLY APPLIED TO THE DETERMINATION OF GLUTATHIONE IN THE PRESENCE OF DIFFERENT CONCENTRATION OF CYSTEINE IN SEVERAL SYNTHETIC MIXTURES.

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APA: Copy

HORMOZI NEZHAD, M.REZA, & MOHAMADI, J.. (2014). SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND CYSTEINE BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER BETWEEN GOLD NANOPARTICLES AND FITC. IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. SID. https://sid.ir/paper/911426/en

Vancouver: Copy

HORMOZI NEZHAD M.REZA, MOHAMADI J.. SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND CYSTEINE BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER BETWEEN GOLD NANOPARTICLES AND FITC. 2014. Available from: https://sid.ir/paper/911426/en

IEEE: Copy

M.REZA HORMOZI NEZHAD, and J. MOHAMADI, “SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND CYSTEINE BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER BETWEEN GOLD NANOPARTICLES AND FITC,” presented at the IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. 2014, [Online]. Available: https://sid.ir/paper/911426/en

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