SIMULTANEOUS DETERMINATION OF GLUCOSE, FRUCTOSE AND SUCROSE IN SOME NATIVE MELONS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND REFRACTOMETRY AND COMPARISON OF THE ANALYTICAL RESULTS

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GHOLAMI ALI; ZAREI HASTI; SOBHANI SAMANEH

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Seminar Paper

Paper Information

Seminar: IRANIAN CHEMISTRY CONGRESS
Year:2013 | Issue:16

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Title

SIMULTANEOUS DETERMINATION OF GLUCOSE, FRUCTOSE AND SUCROSE IN SOME NATIVE MELONS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND REFRACTOMETRY AND COMPARISON OF THE ANALYTICAL RESULTS

Author(s)

GHOLAMI ALI | ZAREI HASTI | SOBHANI SAMANEH | Issue Writer Certificate

Keywords

SOLUBLE SUGARS

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

REFRACTOMETRY

FRUIT SUGAR

NATIVE MELONS

Abstract

DETERMINATION OF SOLUBLE SUGAR CONTENTS IN FRESH FRUITS AND THEIR JUICE PRODUCTS IS AN IMPORTANT FOOD NUTRITION ANALYSIS MEANS FOR EVALUATING QUALITY AND DETECTING ADULTERATION [1, 2]. THE SWEETNESS OF FRUITS, SUCH AS MELONS, IS THE CENTRAL CHARACTERISTIC IN DETERMINING FRUIT QUALITY. MELON SWEETNESS IS DETERMINED BY THE TOTAL SUGAR CONTENT AND BY THE RATIOS AMONG THE MAIN ACCUMULATED SUGARS- GLUCOSE, FRUCTOSE AND SUCROSE [3]. IN THIS STUDY, THE AMOUNT OF THESE SUGARS AS MAJOR CARBOHYDRATES IN NATIVE MELONS WAS MEASURED BY HPLC.UNDER THE OPTIMIZED HPLC CONDITIONS, 3 MONOSACCHARAIDES WERE SEPARATED USING A SHANDON HYPERSIL H5APS COLUMN (250 MM× 4.6 MM) AND ISOCRATIC ELUTION WITH ACETONITRILE/WATER (85: 15) AT FLOW RATE OF 1 ML/MIN. THE STANDARD SOLUTIONS AND SAMPLES WERE PREPARED IN ACN/H2O (50% V/V). THE HPLC METHOD WAS ASSUMED TO PROVIDE THE REFERENCE VALUE OF THE ANALYTE FOR CALIBRATION. THE TOTAL SUGAR CONTENT OF THE SAMPLES WAS DETECTED BY REFRACTOMETRY USING A SIMPLE INSTRUMENT, ARE IN A GOOD AGREEMENT WITH THE TOTAL HPLC VALUES. THE RESULTS FROM BOTH METHODS ARE CRITICALLY EVALUATED AND THEIR IMPACT FOR STUDIES OF AUTHENTICITY IS DISCUSSED.

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APA: Copy

GHOLAMI, ALI, ZAREI, HASTI, & SOBHANI, SAMANEH. (2013). SIMULTANEOUS DETERMINATION OF GLUCOSE, FRUCTOSE AND SUCROSE IN SOME NATIVE MELONS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND REFRACTOMETRY AND COMPARISON OF THE ANALYTICAL RESULTS. IRANIAN CHEMISTRY CONGRESS. SID. https://sid.ir/paper/913593/en

Vancouver: Copy

GHOLAMI ALI, ZAREI HASTI, SOBHANI SAMANEH. SIMULTANEOUS DETERMINATION OF GLUCOSE, FRUCTOSE AND SUCROSE IN SOME NATIVE MELONS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND REFRACTOMETRY AND COMPARISON OF THE ANALYTICAL RESULTS. 2013. Available from: https://sid.ir/paper/913593/en

IEEE: Copy

ALI GHOLAMI, HASTI ZAREI, and SAMANEH SOBHANI, “SIMULTANEOUS DETERMINATION OF GLUCOSE, FRUCTOSE AND SUCROSE IN SOME NATIVE MELONS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND REFRACTOMETRY AND COMPARISON OF THE ANALYTICAL RESULTS,” presented at the IRANIAN CHEMISTRY CONGRESS. 2013, [Online]. Available: https://sid.ir/paper/913593/en

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