PURIFICATION OF BRUCELLA VIRB12 AS A SOLUBLE RECOMBINANT PROTEIN FROM INCLUSION BODIES

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MIRKALANTARI SHIVA; AMIRMOZAFARI NOUR; IRAJIAN GHOLAMREZA

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY
Year:2015 | Issue:16

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Title

PURIFICATION OF BRUCELLA VIRB12 AS A SOLUBLE RECOMBINANT PROTEIN FROM INCLUSION BODIES

Author(s)

MIRKALANTARI SHIVA | AMIRMOZAFARI NOUR | IRAJIAN GHOLAMREZA | Issue Writer Certificate

Keywords

INCLUSION BODY

VIRB12 RECOMBINANT PROTEIN

BRUCELLA

SOLUBLE PROTEIN

PROTEIN PURIFICATION

Abstract

BACKGROUND AND AIM: THE OVER-PRODUCTION OF BRUCELLA VIRB12 RECOMBINANT PROTEIN IN E. COLI RESULTS IN THE FORMATION OF INCLUSION BODIES COMPOSED OF INSOLUBLE AGGREGATES OF THE EXPRESSED PROTEIN. INCLUSION BODIES WERE FORMED IN THE CYTOPLASM. INSOLUBLE AGGREGATES OF MISFOLDED PROTEIN LACKING BIOLOGICAL ACTIVITY AND SHOULD BE CONVERTED TO SOLUBLE PROTEIN FOR EXHIBITING THE DESIRED ACTIVITY. AIM OF THIS STUDY WAS TO MAKE SOLUBILIZE INCLUSION BODIES OF BRUCELLA VIRB12 RECOMBINANT PROTEIN.METHODS: PURE INCLUSION BODIES PREPARED BY LOW SPEED CENTRIFUGATION AFTER CELL LYSIS. THE RESIDUE OF MEMBRANE FRACTION WAS REMOVED BY TRITON X-100. ...

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APA: Copy

MIRKALANTARI, SHIVA, AMIRMOZAFARI, NOUR, & IRAJIAN, GHOLAMREZA. (2015). PURIFICATION OF BRUCELLA VIRB12 AS A SOLUBLE RECOMBINANT PROTEIN FROM INCLUSION BODIES. INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY. SID. https://sid.ir/paper/927170/en

Vancouver: Copy

MIRKALANTARI SHIVA, AMIRMOZAFARI NOUR, IRAJIAN GHOLAMREZA. PURIFICATION OF BRUCELLA VIRB12 AS A SOLUBLE RECOMBINANT PROTEIN FROM INCLUSION BODIES. 2015. Available from: https://sid.ir/paper/927170/en

IEEE: Copy

SHIVA MIRKALANTARI, NOUR AMIRMOZAFARI, and GHOLAMREZA IRAJIAN, “PURIFICATION OF BRUCELLA VIRB12 AS A SOLUBLE RECOMBINANT PROTEIN FROM INCLUSION BODIES,” presented at the INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY. 2015, [Online]. Available: https://sid.ir/paper/927170/en

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