IN VITRO REFOLDING OF NEW RECOMBINANT BACTERIAL LACCASE FROM BACILLUS SP. HR03

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MOLLANIA NASRIN; KHAJEH KHOSRO; Rashno Fatemeh; AKBARI NEDA

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL IRANIAN BIOLOGY CONFERENCE
Year:2012 | Issue:17

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Title

IN VITRO REFOLDING OF NEW RECOMBINANT BACTERIAL LACCASE FROM BACILLUS SP. HR03

Author(s)

MOLLANIA NASRIN | KHAJEH KHOSRO | Rashno Fatemeh | AKBARI NEDA

Keywords

LACCASE

REFOLDING

INCLUSION BODY

INTRINSIC FLUORESCENCE

Abstract

A RECOMBINANT FORM OF LACCASE (BENZENEDIOL OXYGEN OXIDOREDUCTASES, EC 1.10.3.2) FROM BACILLUS SP.HR03 WAS OVEREXPRESSED IN ESCHERICHIA COLI BL-21 (DE3). INCLUSION BODY FORMATION HAPPENS QUITE OFTEN DURING RECOMBINANT PROTEIN PRODUCTION. REFOLDING CONDITIONS TO YIELD ACTIVE ENZYMES-USING DILUTION METHOD -WERE OPTIMIZED TAKING AS A STARTING POINT FOR STUDYING BACTERIAL LACCASE. SOLUBILIZATION OF INCLUSION BODIES WAS CARRIED OUT IN PHOSPHATE BUFFER PH 7, CONTAINING 8 M UREA AND 4 MMB-MERCAPTOETHANOL. THE EFFECT OF DIFFERENT ADDITIVES WAS INVESTIGATED ON THE REFOLDING PROCEDURE OF DENATURATED LACCASE. ALSO KINETIC PARAMETERS OF SOLUBLE AND REFOLDED LACCASE WERE ANALYZED.

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APA: Copy

MOLLANIA, NASRIN, KHAJEH, KHOSRO, Rashno, Fatemeh, & AKBARI, NEDA. (2012). IN VITRO REFOLDING OF NEW RECOMBINANT BACTERIAL LACCASE FROM BACILLUS SP. HR03. INTERNATIONAL IRANIAN BIOLOGY CONFERENCE. SID. https://sid.ir/paper/929100/en

Vancouver: Copy

MOLLANIA NASRIN, KHAJEH KHOSRO, Rashno Fatemeh, AKBARI NEDA. IN VITRO REFOLDING OF NEW RECOMBINANT BACTERIAL LACCASE FROM BACILLUS SP. HR03. 2012. Available from: https://sid.ir/paper/929100/en

IEEE: Copy

NASRIN MOLLANIA, KHOSRO KHAJEH, Fatemeh Rashno, and NEDA AKBARI, “IN VITRO REFOLDING OF NEW RECOMBINANT BACTERIAL LACCASE FROM BACILLUS SP. HR03,” presented at the INTERNATIONAL IRANIAN BIOLOGY CONFERENCE. 2012, [Online]. Available: https://sid.ir/paper/929100/en

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