APPLICATIONS OF INSECT CELL CULTURE AND BACULOVIRUSE FOR RECOMBINANT PROTEIN PRODUCTION: A REVIEW

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HASHEMI NEDA; MORTAZAVI SEYED ALI; GAZERANI SAMANE

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT
Year:2015 | Issue:1

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Title

APPLICATIONS OF INSECT CELL CULTURE AND BACULOVIRUSE FOR RECOMBINANT PROTEIN PRODUCTION: A REVIEW

Author(s)

HASHEMI NEDA | MORTAZAVI SEYED ALI | GAZERANI SAMANE

Keywords

BACULOVIRUSE EXPRESSION VECTORS SYSTEM

INSECT CELL

VACCIN

RECOMBINANT PROTEINS

Abstract

INSECT CELL CULTURES ARE WIDELY USED FOR THE PRODUCTION OF RECOMBINANT PROTEINS, VACCINES AND VIRAL PESTICIDES. IT HAS BECOME A COMMON EXPRESSION SYSTEM FOR BOTH BASIC RESEARCH AND LARGE-SCALE COMMERCIAL APPLICATIONS. THE BACULOVIRUSES EXPRESSION SYSTEM IS BASED ON THE REPLACEMENT OF NON-ESSENTIAL, VIRAL GENE, TERMED POLYHEDRIN, WITH A GENE OF INTEREST. THE INSERTION OF FOREIGN DNA AT THE POLYHEDRIN LOCUS WITHIN THE VIRAL GENOME RESULTS IN INCORPORATION OF THE FOREIGN DNA INTO PROGENY VIRUS PARTICLES AND SUBSEQUENT HIGH-LEVEL EXPRESSION OF THE RECOMBINANT PROTEIN WITHIN EUKARYOTIC INSECT CELL LINES. A MAIN FACTOR TO THE POPULARITY OF INSECT CELL EXPRESSION IS THE ABILITY OF INSECT CELLS TO PRODUCE RELATIVELY LARGE QUANTITIES EUKARYOTIC PROTEINS IN A RELATIVELY SHORT PERIOD OF TIME.IN THIS REVIEW, WE FOCUS ON THE CELL LINE OF INSECTS AND BACULOVIRUSES THAT USED IN BEV SYSTEM AND RECOMBINANT PROTEINS PRODUCTION IN THIS SYSTEM.

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APA: Copy

HASHEMI, NEDA, MORTAZAVI, SEYED ALI, & GAZERANI, SAMANE. (2015). APPLICATIONS OF INSECT CELL CULTURE AND BACULOVIRUSE FOR RECOMBINANT PROTEIN PRODUCTION: A REVIEW. INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT. SID. https://sid.ir/paper/933464/en

Vancouver: Copy

HASHEMI NEDA, MORTAZAVI SEYED ALI, GAZERANI SAMANE. APPLICATIONS OF INSECT CELL CULTURE AND BACULOVIRUSE FOR RECOMBINANT PROTEIN PRODUCTION: A REVIEW. 2015. Available from: https://sid.ir/paper/933464/en

IEEE: Copy

NEDA HASHEMI, SEYED ALI MORTAZAVI, and SAMANE GAZERANI, “APPLICATIONS OF INSECT CELL CULTURE AND BACULOVIRUSE FOR RECOMBINANT PROTEIN PRODUCTION: A REVIEW,” presented at the INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT. 2015, [Online]. Available: https://sid.ir/paper/933464/en

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