Inhibitory Effect of miRNA-145 on PD-L1 Expression in Breast Cancer Cell Line

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Hajibabaei s.; Sotoodehnejadnematalahi F.; NAFISI N.; ZEINALI S.; AZIZI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Breast Diseases Year:2022 | Volume:14 | Issue:4 (55) Page(s): 45-61

Download Full-Text

Persian Verion

View:

209

Download:

191

Cites:

Information Journal Paper

Title

Inhibitory Effect of miRNA-145 on PD-L1 Expression in Breast Cancer Cell Line

Author(s)

Keywords

PD-L1

miRNA-145

Breast Cancer

Abstract

Introduction: PD-L1 is one of the most important immune control molecules in Breast Cancer and plays an important role in suppressing the immune system against tumor cells. Controlling the expression of PD-L1 at mRNA level using miRNA inhibitors could be helpful strategy for cancer treatment. In this study, considering the possible role of miR-145 as a tumor suppression in Breast Cancer, its involvement in reducing PD-L1 expression in Breast Cancer cell lines has been investigated. Methods: First, the targeting of miRNA-145 on 3 'UTR of PD-L1 gene was confirmed using bioinformatics software and then by luciferase dual reporter assay. The expression level of miRNA-145 was measured in Breast Cancer cell lines compared to normal line. After transfection of miRNA-145 into Breast Cancer cell lines, qRTPCR was used to evaluate the effect of miRNA-145 on PD-L1 expression. Results: we showed that decreased expression of miRNA-145 in Breast Cancer cell lines was directly related to increased PD-L1 expression (r =-0. 6175, P value₌ 0. 0457). In addition, increased expression of miRNA-145 in Breast Cancer cell lines MDA-MB231, BT549 and MCF7 significantly reduced PD-L1 expression (1. 938± 0. 212, 1. 784± 0. 03 and 0. 083± 0. 02 respectively). Conclusion: Our findings suggest that miRNA-145, by targeting the PD1/PD-L1 pathway and reducing PD-L1 expression, may be a therapeutic agent to prevent the progression of Breast Cancer.

Cites

No record.

References

No record.

Cite

APA: Copy

Hajibabaei, s., Sotoodehnejadnematalahi, F., NAFISI, N., ZEINALI, S., & AZIZI, M.. (2022). Inhibitory Effect of miRNA-145 on PD-L1 Expression in Breast Cancer Cell Line. IRANIAN QUARTERLY JOURNAL OF BREAST DISEASE, 14(4 (55) ), 45-61. SID. https://sid.ir/paper/952725/en

Vancouver: Copy

Hajibabaei s., Sotoodehnejadnematalahi F., NAFISI N., ZEINALI S., AZIZI M.. Inhibitory Effect of miRNA-145 on PD-L1 Expression in Breast Cancer Cell Line. IRANIAN QUARTERLY JOURNAL OF BREAST DISEASE[Internet]. 2022;14(4 (55) ):45-61. Available from: https://sid.ir/paper/952725/en

IEEE: Copy

s. Hajibabaei, F. Sotoodehnejadnematalahi, N. NAFISI, S. ZEINALI, and M. AZIZI, “Inhibitory Effect of miRNA-145 on PD-L1 Expression in Breast Cancer Cell Line,” IRANIAN QUARTERLY JOURNAL OF BREAST DISEASE, vol. 14, no. 4 (55) , pp. 45–61, 2022, [Online]. Available: https://sid.ir/paper/952725/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops