مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

456
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Osteoblast Differentiation of Human Dental Pulp Stem Cells with Dexamethasone insitu Forming Implant

Pages

  70-82

Abstract

 Introduction: Dental pulp stem cells (DPSCs) are known as multipotent stem cells which differentiate into three different cell lines, including osteocytes, chondrocytes, and adipocytes. Until now, dental precursor cells have been used in tissue engineering studies for pre-clinical applications. The Dexamethasone in-situ forming implant (ISFI) is capable of releasing medicine that is trapped in the aquatic environment slowly and continuously in a few days to weeks. The present research aimed to investigate the ability of human DPSCs to differentiate into bone tissue in the presence of dexamethasone ISFI as a source of dental Mesenchymal stem cells. Materials and Methods: The DPSCs were extracted from the third molar tooth. To investigate the process of differentiation into osteoblasts, β-glycerophosphate, ascorbic acid, and dexamethasone ISFI in solution were added to the cells. The differentiation process was verified by Alizarin Red S stain, alkaline phosphatase activity (ALP), and western blot technique. Results: Based on the results of Alizarin Red S staining, extracellular matrix mineralization was increased in dexamethasone ISFI (i. e., implant) and standard (i. e., dexamethasone) groups. In addition, the ALP activity underwent an increase in these groups, compared to the related control group. Furthermore, the protein level of ALP, osteopontin, and runt-related transcription factor two increased in the group treated with dexamethasone ISFI, compared to the control group. Conclusion: Findings of this study indicated that dexamethasone ISFI could increase the process of differentiation of DPSCs into osteoblasts.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    TAYARANI NAJARAN, ZAHRA, KAMALI, HOSSEIN, HADIZADEH, FARZIN, AHMADI, FARAHNAZ, Shahidi, Sahar, & KHODAVERDI, ELHAM. (2021). Osteoblast Differentiation of Human Dental Pulp Stem Cells with Dexamethasone insitu Forming Implant. JOURNAL OF MASHHAD DENTAL SCHOOL, 45(1 ), 70-82. SID. https://sid.ir/paper/953658/en

    Vancouver: Copy

    TAYARANI NAJARAN ZAHRA, KAMALI HOSSEIN, HADIZADEH FARZIN, AHMADI FARAHNAZ, Shahidi Sahar, KHODAVERDI ELHAM. Osteoblast Differentiation of Human Dental Pulp Stem Cells with Dexamethasone insitu Forming Implant. JOURNAL OF MASHHAD DENTAL SCHOOL[Internet]. 2021;45(1 ):70-82. Available from: https://sid.ir/paper/953658/en

    IEEE: Copy

    ZAHRA TAYARANI NAJARAN, HOSSEIN KAMALI, FARZIN HADIZADEH, FARAHNAZ AHMADI, Sahar Shahidi, and ELHAM KHODAVERDI, “Osteoblast Differentiation of Human Dental Pulp Stem Cells with Dexamethasone insitu Forming Implant,” JOURNAL OF MASHHAD DENTAL SCHOOL, vol. 45, no. 1 , pp. 70–82, 2021, [Online]. Available: https://sid.ir/paper/953658/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top