Recombinant Expression of Hemagglutinin Protein of Iranian Swine influenza A (H1N1) in the Insect Cells Using Baculovirus System

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Zahmati Sara; MAHDAVI MEHDI; Taghizadeh Tarnabi Morteza; HAGHIGHAT SETAREH; Jalalirad Reza

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2021 | Volume:39 | Issue:617 Page(s): 181-188

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Title

Recombinant Expression of Hemagglutinin Protein of Iranian Swine influenza A (H1N1) in the Insect Cells Using Baculovirus System

Author(s)

Keywords

Influenza A virus

H1N1 subtype

Hemagglutinin

Insecta

Baculoviridae

Vaccines

Abstract

Background: Influenza virus, which is a decisive agent of influenza, attracted interest because of the event of the annual epidemic among a wide range of animal and human hosts. This study aimed to express the Hemagglutinin protein of Iranian swine Influenza A (H1N1) through a baculovirus system in SF9 cells to produce a new recombinant vaccine. Methods: Hemagglutinin gene of Swine H1N1 virus was amplified with specific primers containing restriction enzymes site, and then cloned. Afterward, the vector was transformed to DH10Bac using Bac-to-Bac system in order to produce a recombinant bacmid. Hemagglutinin expression and its biological activity were assessed using molecular and immunization tests. Findings: The target rHA in length, 1710 bp, was produced and expressed in transfected SF9 cells with a size of ~66 kDa. The infected cells expanded in size and their nucleus, and desiccated from the surface of the cell culture as a granular. They could absorb chick red blood cells (RBCs), and appear as cell aggregates forty-eight post-infection. The fact that infected cells were unable to form cell clamp showed the test's accuracy and inhibition of hemodesorption activity. The amount of protein obtained was 10. 76 μ g/100 μ l, equal to 0. 1 mg/ml. Conclusion: The baculovirus expression system could express the recombinant protein in the insect cell. Therefore, it may be a well-suited alternative to produce a new generation of the vaccine instead of egg-based and cell-culture-based generation Vaccines.

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APA: Copy

Zahmati, Sara, MAHDAVI, MEHDI, Taghizadeh Tarnabi, Morteza, HAGHIGHAT, SETAREH, & Jalalirad, Reza. (2021). Recombinant Expression of Hemagglutinin Protein of Iranian Swine influenza A (H1N1) in the Insect Cells Using Baculovirus System. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 39(617 ), 181-188. SID. https://sid.ir/paper/954966/en

Vancouver: Copy

Zahmati Sara, MAHDAVI MEHDI, Taghizadeh Tarnabi Morteza, HAGHIGHAT SETAREH, Jalalirad Reza. Recombinant Expression of Hemagglutinin Protein of Iranian Swine influenza A (H1N1) in the Insect Cells Using Baculovirus System. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2021;39(617 ):181-188. Available from: https://sid.ir/paper/954966/en

IEEE: Copy

Sara Zahmati, MEHDI MAHDAVI, Morteza Taghizadeh Tarnabi, SETAREH HAGHIGHAT, and Reza Jalalirad, “Recombinant Expression of Hemagglutinin Protein of Iranian Swine influenza A (H1N1) in the Insect Cells Using Baculovirus System,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 39, no. 617 , pp. 181–188, 2021, [Online]. Available: https://sid.ir/paper/954966/en

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