The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line

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Kavousi Mahsa; Fatemimoghadam Delnaz

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Journal Paper

Paper Information

Journal: Pars Journal of Medical Sciences Year:2019 | Volume:17 | Issue:3 Page(s): 17-25

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Title

The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line

Author(s)

Kavousi Mahsa | Fatemimoghadam Delnaz | Issue Writer Certificate

Keywords

Brain Cancer

Bcl-2

Real-time PCR

MTT

Spirulina

Abstract

Introduction: U87 cell line contains glioblastoma cancer cells as the most acute type of human brain tumor. Increased expression of Bcl-2 gene in cancer cells has anti-apoptotic effect. In the present study the effect of Spirulina extract on gene expression in cancer cell line was investigated. Materials and Methods: In this in laboratory study, the U87 cell line was used. The Brain Cancer cell line was procured and cultivated from the Iranian Biological Resource Center. Cells were divided into two treatment and control groups. Spirulina alga extract was affected on the treatment group and its toxicity was measured using MTT assay (compound 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromidefor). The reference gene in this study was GAPDH. RNA was extracted from control and control cells and cDNA was made. The expression of genes was evaluated using ∆ ∆ Ct in Real-time PCR. Results: The result of MTT assay showed that the IC50 (Median Inhibition Concentration) value was 10. 01 mg/ml for the 48 hour treatment. The result of Real-time PCR showed that the expression of Bcl-2 and the anti-apoptotic gene significantly decreased 3 folds in treatment group. Conclusion: According to the results of MTT assay and Real-time PCR, the expression of Bcl-2 significantly decreased 3 times in treatment group.

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APA: Copy

Kavousi, Mahsa, & Fatemimoghadam, Delnaz. (2019). The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 17(3 ), 17-25. SID. https://sid.ir/paper/961643/en

Vancouver: Copy

Kavousi Mahsa, Fatemimoghadam Delnaz. The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)[Internet]. 2019;17(3 ):17-25. Available from: https://sid.ir/paper/961643/en

IEEE: Copy

Mahsa Kavousi, and Delnaz Fatemimoghadam, “The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line,” PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), vol. 17, no. 3 , pp. 17–25, 2019, [Online]. Available: https://sid.ir/paper/961643/en

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