Introduction of flow cytometric analysis method (Part 1: principle and components of the flow cytometer)

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Anaam Marzieh; Sotoudeh Sharareh; chamaei nejad Forough; Nejadmoghaddam Mohammad Reza

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF LABORATORY KNOWLEDGE Year:2021 | Volume:9 | Issue:2 (34) Page(s): 53-60

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Information Journal Paper

Title

Introduction of flow cytometric analysis method (Part 1: principle and components of the flow cytometer)

Author(s)

Anaam Marzieh | Sotoudeh Sharareh | chamaei nejad Forough | Nejadmoghaddam Mohammad Reza | Issue Writer Certificate

Keywords

Flowcytometer

Cell

Size

Granularity

Fluorescence dye

Abstract

Flowcytometery is an efficient technique in cytology that is capable of simultaneously measuring and analyzing multiple physical properties such as Size and Granularity of a single Cell or any other soluble particle. In addition, the efficiency of this technique depends on the light scattered features of Fluorescence dyes or conjugated monoclonal antibody fluorescence that bind to the target molecule on surface or inside the Cell. The Flowcytometer components, including fluidics system, optics (laser and filters), signal processor, software and electrostatic Cell sorting, together provide a powerful tool for accurate analysis of complex Cell populations in a short time. Since this technique is widely used in various fields such as medical diagnosis, immunology, pathology, molecular biology, plant science, microbiology, etc., it is important to understand the components and function of flow cytometry.

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APA: Copy

Anaam, Marzieh, Sotoudeh, Sharareh, chamaei nejad, Forough, & Nejadmoghaddam, Mohammad Reza. (2021). Introduction of flow cytometric analysis method (Part 1: principle and components of the flow cytometer). IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, 9(2 (34) ), 53-60. SID. https://sid.ir/paper/962213/en

Vancouver: Copy

Anaam Marzieh, Sotoudeh Sharareh, chamaei nejad Forough, Nejadmoghaddam Mohammad Reza. Introduction of flow cytometric analysis method (Part 1: principle and components of the flow cytometer). IRANIAN JOURNAL OF LABORATORY KNOWLEDGE[Internet]. 2021;9(2 (34) ):53-60. Available from: https://sid.ir/paper/962213/en

IEEE: Copy

Marzieh Anaam, Sharareh Sotoudeh, Forough chamaei nejad, and Mohammad Reza Nejadmoghaddam, “Introduction of flow cytometric analysis method (Part 1: principle and components of the flow cytometer),” IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, vol. 9, no. 2 (34) , pp. 53–60, 2021, [Online]. Available: https://sid.ir/paper/962213/en

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